Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 226-641-8 | CAS number: 5444-75-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 May - 27 May 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted in 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- adopted in 1992
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 72-1 (Fish Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Duplicate medium samples (100 mL) were taken for analysis from each replicate vessel of the solvent control and the test concentration at 0, 24, 72 and 96 h.
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was dissolved in tetrahydrofuran (THF) to give a stock solution of 5.0 mg/L. This stock solution was dispensed automatically by the dosing apparatus into the flowing test water to give the desired test concentrations. The solvent stock solution was prepared twice during the exposure period and stored at approx. 4 °C until required.
- Controls: Diluent water with 100 µL THF/L
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tetrahydrofuran
- Evidence of undissolved material (e.g. precipitate, surface film, etc): At 72 and 96 hours a layer of undissolved test compound was noted on the water surface in all the replicate exposure vessels. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Fish were obtained from Donnington Fish Farm, Stow-on-the-Wold, UK on 9 May 2000.
- Length at study initiation: 4.4 cm (SD = 0.1 cm)
- Weight at study initiation: 1.26 g (SD = 0.16 g)
- Feeding during test: None
ACCLIMATION
- Acclimation period: 15 - 23 May 2000
- Acclimation conditions (same as test or not): 14 - 17 °C, 8.1 - 9.2 mg O2/L
- Type and amount of food: Fry pellets
- Feeding frequency: Daily until 48 h before test start
- Health during acclimation (any mortality observed): No mortality occurred in the 7 days before test start. - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 157 - 187 mg/L CaCO3
- Test temperature:
- 15 °C constant
- pH:
- 7.5 - 7.9
- Dissolved oxygen:
- 8.6 - 9.7 mg/L
- Nominal and measured concentrations:
- Nominal: 0 and 0.5 mg/L
Measured: < LOD, 0.66 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Aquarium
- Material, size, fill volume: Glass, 25 x 46 x 25 cm, 20 L
- Aeration: No aeration of the aquaria, but aeration of the dilution water before delivery to the test system
- Renewal rate of test solution (frequency/flow rate): 260 - 300 mL/min
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3
- Biomass loading rate: 0.63 g/L (static volume) and 0.034 g/L of test solution passing through the chamber in 24 h
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water, filtered, dechlorinated, softened, reverse osmosis treated
- Pesticides: All measured pesticides were < 0.02 µg/L.
- Chlorine: 0.01 - 0.08 mg/L
- Alkalinity: 110 - 116 mg/L CaCO3
- Conductivity: 220 - 223 µS
- Culture medium different from test medium: No
- Intervals of water quality measurement: Temperature, pH and oxygen were recorded daily for each vessel. Alkalinity and conductivity were measured at test start and test end in all vessels.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Mortality was observed at 15 min, 3, 6, 24, 48, 72 and 96 h.
- Sublethal effects were also recorded. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.66 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: One fish escaped from one of the solvent control tanks.
- Mortality or adverse effects in the test solution: None observed
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: At 72 and 96 hours a layer of undissolved test compound was noted on the water surface in all the replicate exposure vessels. In replicate 3 it was believed the layer was disturbed during sampling and dispersed into the water column resulting in a non-homogenous sample containing increased test material. The layer of undissolved test material is considered to have formed as a result of the continuous addition of the test substance to already saturated test medium.
- Effect concentrations exceeding solubility of substance in test medium: Yes
- Analytics: Measured concentrations ranged from 69 - 85% of nominal at 0 h, 75 - 93% of nominal at 24 h, 98 - 356% of nominal at 72 h and 92 - 325% of nominal at 96 h.
Reference
Description of key information
No adverse effects up to the limit of water solubility (0.4 mg/L measured in aqua dest; 0.66 mg/L in fish test medium) for Oncorhynchus mykiss (OECD 203)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.66 mg/L
Additional information
Short-term toxicity of 2-ethylhexyl benzoate to fish was investigated in one study with rainbow trout (Oncorhynchus mykiss) according to OECD guideline 203 and GLP (Barrett, 2001). Three replicates of ten juvenile fish were exposed to a single concentration of the test substance of nominally 0.5 mg/L dispersed in water. This concentration was determined to be the limit of solubility for the test substance under the conditions of this study. Three replicate control and solvent control vessels were also prepared. The measured concentrations ranged from 69% - 85% at test start to 92% - 325% at test end. The latter value is assumed to be the result of the continuous addition of the test substance to an already saturated test medium (a layer of undissolved test material was noted from 72 hours on the water surface). The mean measured concentration was thus calculated as 0.66 mg/L. As no mortality was observed in these saturated solutions throughout the study, the LC0 (96 h) and LC50 (96 h) were assumed as 0.66 mg/L and > 0.66 mg/L (based on mean measured concentrations), respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.