2,2-bis(hydroxymethyl)propionic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993-08-30 - 1993-09-02

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

- Name of the test material used in the study report: 2.2-bis(hydroxymethyl)propionic acid
- Batch no.: 63004-14

Analytical monitoring:

yes

Details on sampling:

Portions of the stock and test solutions were sampled and delivered to the sponsor for analysis.

Vehicle:

no

Details on test solutions:

Not applicable

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish

ACCLIMATION
- Acclimation period: 14 days in internal quarantine.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not applicable

Hardness:

250 ± 25 mg calcium carbonate/L (nominal value)

Test temperature:

24±1ºC

pH:

6.5 - 8.7 (recorded values)

Dissolved oxygen:

The dilution water was aerated to oxygen saturation exceeding 90%.
The dissolved oxygen concentration in the test vessels was at least 60% of the air saturation value and ranged between 60 - 100 %.

Salinity:

Not applicable

Nominal and measured concentrations:

0, 100, and 1000 mg/L (nominal values)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: not specified
- Material, size, headspace, fill volume: 5 L glass vessels
- Aeration: test solutions were aerated prior to use (>90% oxygen saturation)
- Renewal rate of test solution (frequency/flow rate): test solutions were renewed every 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.6 grams of fish per litre


OTHER TEST CONDITIONS
- Adjustment of pH: Not reported, although adjustment (to pH 7.8 ± 0.2) permitted using NaOH or HCl as appropriate.
- Photoperiod: 16 hour light, 8 hours dark.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No treatment related mortalities occurred during the test period.

Results with reference substance (positive control):

The sensitivity of the fish population used for potassium dichromate was 366 mg/L which is in accordance with the Swedish Standard SS 02 81 62

Reported statistics and error estimates:

Not applicable

Validity criteria fulfilled:

yes

Conclusions:

In a short term toxicity test with fish (Danio rerio), no mortalities were observed and the LC50 value over the 96 hour test period was >1000 mg/L (based on nominal exposure concentrations).

Executive summary:

In a 96-h acute toxicity study, zebrafish (Danio rerio) were exposed to Bis-MPA at nominal concentrations of 0, 100 and 1000 mg/L under semi-static conditions.  The 96-h LC₅₀ was >1000 mg/L.   

This toxicity study is classified as acceptable and satisfies the guideline requirement for an acute fish toxicity study.

 Results Synopsis

Test Type: Static Renewal

LC₅₀:  >1000 mg/L                        

Endpoint Effected: Mortality

Description of key information

In a short term toxicity test with fish (Danio rerio), no mortalities were observed and the LC50 value over the 96 hour test period was >1000 mg/L (based on nominal exposure concentrations).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 1 000 mg/L