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Diss Factsheets

Administrative data

Key value for chemical safety assessment

Additional information

In a bacterial reverse mutation assay conducted with methods similar to OECD 471 guidelines, S. typhimurium strains TA98, TA100, TA1535 and TA1538 were treated with ethanol, 2-(octylthio) (96.9%) at concentrations of 0, 3, 10, 33 and 333 µg/plate in the presence and absence of rat liver S9 activation. No induced mutations were observed in the indicator strains in either the presence or absence of metabolic activation. At doses above 100 µg/plate, in the absence of activation, toxicity was moderate to extreme (U. S. EPA, 1995; National Industrial Chemicals Notification and Assessment Scheme, 1998). In another bacterial reverse mutation assay conducted similar to OECD 471 guidelines, 2-(octylthio)ethanol was not mutagenic to S. typhimurium strains TA 98, TA 100, TA1535 or TA1537 in the presence or absence of metabolic action at doses up to 100 µg/plate (Hazleton Laboratories, Inc., 1984).

In vitro mammalian cell gene mutation assays were conducted using L5178Y TK +/- or CHO cells. In the L5178Y TK +/- mouse lymphoma assay conducted with methods similar to OECD 476 guidelines results indicated that 2 -(octylthio)ethanol was non-mutagenic in both the presence and absence of metabolic activation (U.S., EPA, 1995; National Industrial Chemicals Notification and Assessment Scheme, 1998; Hazleton Laboratories America Inc., 1983). 2-(octylthiol)ethanol was also not mutagenic (endpoint not specified) in CHO cells in the presence or absence of metabolic activation (U.S. EPA, 1995).

In a chromosome aberration assay conducted with methods similar to OECD guideline 407, Chinese Hamster Ovary (CHO) cells were exposed for 10 hours to ethanol, 2-(octylthio) at concentrations ranging from 0.007-0.075 µL/mL in the absence of metabolic activation, and for 2 hours at concentrations ranging from 0.013-0.15 µL/mL in the presence of rat liver S9 activation.  The test substance failed to induce structural chromosomal aberrations in either the presence or absence of metabolic activation, and was therefore considered non-mutagenic in the assay (National Industrial Chemicals Notification and Assessment Scheme, 1998). There were no increases in sister chromatid exchanges (SCEs) when CHO cells were treated with 2-(octythio)ethanol in the presence or absence or metabolic activation (Hazleton Laboratories America Inc., 1984).

2-(octylthio)ethanol did not induce an increase in unscheduled DNA synthesis in rat primary hepatocytes (U.S. EPA, 1995; National Industrial Chemicals Notification and Assessment Scheme, 1998). 


Short description of key information:
2-(octylthiol)ethanol has been examined for genotoxicity in a range of recognized core in vitro assay types and has shown negative results.

Endpoint Conclusion:

Justification for classification or non-classification

2-(octylthiol)ethanol has been examined for genotoxicity in a range of recognized core in vitro assay types and has shown negative results. Therefore, 2-(octylthio)ethanol does not warrant classification as a mutagen under DSD or CLP.