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REACH

3,5,5-trimethylhexan-1-ol

EC number: 222-376-7 | CAS number: 3452-97-9

Life Cycle description
Uses advised against

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: OECD 2002, Part 2; Daphnia Reproduction Test

Principles of method if other than guideline:

GLP guideline study. However, according to OECD TG 202 the exposure concentration selection should be based on the EC50 (24 h) for immobilization, starting at about the 24 h EC50 and ending at about 1/100 of the 24 h EC50. The effects of the test concentrations were far lower.

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Regarding the entire test area (1 sample/ 1 test area made by mixing the extracts from the contents of the 4 test containers), after removing the feed by centrifugal separation (at 3000 rpm for 10 minutes) on the exposure start date (0th day), 7th day, 14th day and the exposure end day (21st day), it was analysed by GC by diluting as well as by keeping as-is.

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock Solutions Preparations and Stability: A proportional diluter system was used for intermittent introduction of test material solution (0.1 wt% of test chemical with solubilizer hydrogenated caster oil HCO-40 5000mg/L) and dilution and water into the test chambers.
- Controls: water and vehicle controls included
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): hydrogenated caster oil HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 25 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no data

Test organisms (species):

Daphnia magna

Details on test organisms:

Age at study initiation: < 24 hours after hatching

Test type:

flow-through

Water media type:

freshwater

Limit test:

Total exposure duration:

21 d

Post exposure observation period:

none

Hardness:

55.6mg/L as CaCO3

Test temperature:

19-21 °C

pH:

7.7

Dissolved oxygen:

> 60% of saturation

Nominal and measured concentrations:

Nominal: 0, 0.128. 0.32, 0.8, 2.0, 5.0 mg/L
Measured: 0, 0.904, 0.2298, 0.641, 1.46, 3.87 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): no data whether open / closed
- Material, size, headspace, fill volume: 1.8 L test solution in 3.0L glass vessel (diameter = 16 cm, depth = 17 cm )
- Aeration: no data
- Type of flow-through (e.g. peristaltic or proportional diluter): proportional diluter
- Renewal rate of test solution (frequency/flow rate): flow rate 50 mL/min
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: 180 mL/animal

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Chlorine: <0.02 mg/L
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16h:8h light-dark cycle
- Light intensity: room light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality of parental animals

VEHICLE CONTROL PERFORMED: yes

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

> 3.87 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

21 d

Dose descriptor:

other: ErC50 (reproduction)

Effect conc.:

2.09 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: 95% CL: 1.94-2.25 mg/L

Nominal/measured Concentration:

Measured concentration (mg/L)

(percentage of nominal)

Nominal

concentration

(mg/L)

0-day

7-day

14-day

21-day

Mean*

control

n.d.

Solvent control

n.d.

0.128

0.103

(80.1)

0.0854

(66.7)

0.0878

(68.6)

0.0860

(67.2)

0.0904

(70.7)

0.320

0.273

(85.4)

0.230

(71.9)

0.231

(66.5)

0.200

(62.5)

0.229

(71.5)

0.800

0.703

(87.9)

0.622

(77.7)

0.785

(98.1)

0.454

(56.8)

0.641

(80.1)

2.00

1.62

(80.8)

1.54

(76.8)

1.51

(75.3)

1.19

(59.3)

1.46

(73.0)

5.00

4.08

(81.6)

4.06

(81.2)

3.67

(73.5)

3.67

(73.5)

3.87

(77.4)

n.d. : <0.100 mg

*The values are express as arithmetic means calculated

Water Temperature: 19.9-20.3 degree C

Water Chemistry in test: pH 7.3-7.6; Disooved oxygen = 8.3-8.9 mg/L(Oxygen saturation level >=60%)

Effect Data

Mortality: 21 day LC50 > 3.87 mg/L

Reproduction: 21 day ErC50 = 2.09 mg/L (95% c.i.: 1.94 - 2.25 mg/L); NOECr = 1.46 mg/L; LOECr = 3.87 mg/L

Cumulative number of dead parental Daphnia:

Cumulative number of dead parental Daphnia

Exposure time (day)

Measured

conc.

(mg/L)

control

(0)

(2.5)

(5.0)

(10.0)

(12.5)

(15.0)

Solvent control

(0)

(2.5)

(5.0)

(12.5)

(17.5)

0.0904

(0)

(2.5)

(5.0)

(10.0)

0.229

(0)

(2.5)

(7.5)

(20.0)

(30.0)

(32.5)

0.641

(0)

(2.5)

(5.0)

(12.5)

1.46

(0)

(2.5)

(5.0)

(7.5)

(10.0)

(12.5)

3.87

(0)

(7.5)

(10.0)

(12.5)

The value in parentheses express mortality (%) of Daphnia

Time (days) required to first brood production during exposure

	Vessel No.
Measured concentration (mg/L)	1	2	3	4	Mean*
control	7	7	7	7	7.0
0.0904	7	7	7	7	7.0
0.229	7	7	7	7	7.0
0.641	7	7	7	7	7.0
1.46	7	7	7	7	7.0
3.87	10	10	10	10	10.0

Mean cumulative number of Juvenile produced per adult during exposure:

	Exposure time (day)
Measured conc. (mg/L)	1	2	3	4	5	6	7	8	9	10	11	12
control	0	0	0	0	0	0	4.4	4.4	4.4	38.0	38.0	42.6
Solvent control	0	0	0	0	0	0	5.3	5.3	5.3	38.0	38.0	41.0
0.0904	0	0	0	0	0	0	9.4	9.4	9.4	45.7	45.7	52.0
0.229	0	0	0	0	0	0	7.4	7.4	7.4	40.7	40.7	47.7
0.641	0	0	0	0	0	0	7.6	7.6	7.6	46.9	46.9	53.3
1.46	0	0	0	0	0	0	5.8	5.8	5.8	34.3	34.3	40.1
3.87	0	0	0	0	0	0	0	0	0	3.0	3.0	6.0

	Exposure time (day)
Measured conc. (mg/L)	13	14	15	16	17	18	19	20	21
control	42.6	85.0	85.0	85.0	138	138	188	188	197
Solvent control	41.0	76.4	76.4	76.4	129	129	171	171	185
0.0904	52.0	90.4	90.4	90.4	143	143	188	188	208
0.229	47.7	89.8	89.8	89.8	133	133	176	176	206
0.641	53.3	88.8	88.8	88.8	149	149	174	174	193
1.46	40.1	60.0	60.0	60.0	108	108	128	128	135
3.87	6.0	15.2	15.2	15.2	17.4	17.4	17.5	17.5	23.1

Validity criteria fulfilled:

yes

Conclusions:

According to OECVDT TG 202 the exposure concentration selection should be based on the EC50 (24 h) for immobilization, starting at about the 24 h EC50 and ending at about 1/100 of the 24 h EC50. The effects of the test concentrations were far lower. As to reproduction, the effect values were:
Reproduction: 21 day EC50 = 2.09 mg/L (95% c.i.: 1.94 - 2.25 mg/L); NOECr = 1.46 mg/L; LOECr = 3.87 mg/L

Executive summary:

3, 5,5 -trimethylhexan-1-ol was tested in a Daphnia reproduction test according to OECD TG 202, Part 2, under GLP for 21 days at concentrations up to nominal 5 mg/L (measured 3.87 mg/L). The effect data are as follows:

Mortality: 21 day LC50 > 3.87 mg/L

Reproduction: 21 day ErC50 = 2.09 mg/L (95% c.i.: 1.94 - 2.25 mg/L); NOECr = 1.46 mg/L; LOECr = 3.87 mg/L

According to OECD TG 202 the exposure concentration selection should be based on the EC50 (24 h) for immobilization, starting at about the 24 h EC50 and ending at about 1/100 of the 24 h EC50. The effects of the test concentrations were far lower (CITI, 1997).

Description of key information

3,5,5-trimethylhexan-1-ol was tested in a Daphnia reproduction test according to OECD TG 202, Part. The NOECr was 1.46 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Effect concentration:: 1.46 mg/L

Additional information

3, 5,5 -trimethylhexan-1 -ol was tested in a Daphnia reproduction test according to OECD TG 202, Part 2, under GLP for 21 days at concentrations up to nominal 5 mg/L (measured 3.87 mg/L). The effect data are as follows:

Mortality: 21 day LC50> 3.87 mg/L

Reproduction: 21 day ErC50= 2.09 mg/L (95% c.i.: 1.94 - 2.25 mg/L); NOECr= 1.46 mg/L; LOECr= 3.87 mg/L

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