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EC number: 221-974-5 | CAS number: 3301-94-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 Apr 2018 - 14 Jun 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 6-hexyltetrahydro-2H-pyran-2-one
- EC Number:
- 211-915-1
- EC Name:
- 6-hexyltetrahydro-2H-pyran-2-one
- Cas Number:
- 710-04-3
- Molecular formula:
- C11H20O2
- IUPAC Name:
- 6-hexyloxan-2-one
- Test material form:
- liquid
- Details on test material:
- Appearance: Clear colourless to pale yellow liquid
Test item storage: In refrigerator (2-8°C)
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Preparation of inoculum for exposure: The freshly obtained sludge was kept under continuous aeration until further treatment. Before use, the sludge was coarsely sieved (1 mm) and washed with mineral medium.
- Concentration of sludge: After pretreatment, the concentration of suspended solids (SS) was determined to be 3 g/L in the concentrated sludge as used for the test. The sludge was used as inoculum at a concentration of 1 mL per litre of mineral medium, leading to a final concentration SS of 3 mg/L. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 30.6 mg/L
- Based on:
- test mat.
- Initial conc.:
- 80 mg/L
- Based on:
- ThOD
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium, according to OECD301
- Test temperature: 22 - 24 °C
- pH: 7.8 - 7.9 at start, 7.7 - 7.8 at day 28
- pH adjusted: no
- Continuous aeration: yes
- Continuous darkness: yes
- Other: continuous stirring.
TEST SYSTEM
- Culturing apparatus: 500 mL brown colored glass bottles, fill volume 360 or 244 mL, in order to obtain the required headspace-to-volume ratio.
- Number of culture flasks/concentration: 2 containing test item and inoculum; 2 containing only inoculum (inoculum blank); 1 containing reference item and inoculum (procedure control); 1 containing test item, reference item and inoculum (toxicity control).
- Preparation of bottles: At the start of the test (day 0), test and/or reference item were added to the respective bottles. The volumes of suspensions were made up to 360 mL or 244 mL using inoculated mineral medium.
Rubber gaskets containing two pellets of sodium hydroxide were inserted into each bottle. Subsequently, each bottle was sealed by screwing an automated respirometer head on top.
- Method used to create aerobic conditions: continuous aeration
- Measuring equipment: Lovibond BD600-GLP manometric respirometry system, equipped with an inductive stirring system.
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: The CO2 produced in each test bottle reacted with the CO2 absorbent (Sodium hydroxide pellets) in the rubber gaskets.
MEASUREMENTS
- Measurements were recorded on day 0-3-7-11-14-18-21-25-28. After recording the oxygen consumption on day 28, the pH was determined in all test vessels.
CONTROL AND BLANK SYSTEM
- Abiotic sterile control: no
Reference substance
- Reference substance:
- other: 1-octanol
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 80
- Sampling time:
- 28 d
- Remarks on result:
- other: Bottle A: 85%; Bottle B: 75%.
- Details on results:
- The relative biodegradation values calculated from the measurements performed during the test period revealed 85% and 75% biodegradation of 6-hexyltetrahydro-2H-pyran-2-one (delta undecalactone), for A and B, respectively (based on ThOD). Furthermore, biodegradation of 6-hexyltetrahydro-2H-pyran-2-one (delta undecalactone) of at least 60% was reached within a 10-day window.
In the toxicity control, more than 25% biodegradation occurred within 14 days (79%, based on ThOD). Therefore, the test item was assumed not to inhibit microbial activity.
BOD5 / COD results
- Results with reference substance:
- Functioning of the test system was checked by testing the reference item 1-octanol, which showed a normal biodegradation curve (88% biodegradation within 14 days).
Any other information on results incl. tables
Table 1: Comparison of Biodegradation of the Test Item in Bottles A and B
Day |
Biodegradation (%) |
|||
Bottle A |
Bottle B |
Mean A and B |
∆ A-B1) |
|
0 |
1 |
0 |
1 |
1 |
3 |
33 |
34 |
33 |
1 |
7 |
68 |
66 |
67 |
1 |
11 |
76 |
73 |
74 |
3 |
14 |
79 |
74 |
77 |
5 |
18 |
79 |
74 |
76 |
5 |
21 |
79 |
74 |
76 |
5 |
25 |
84 |
75 |
79 |
9 |
28 |
85 |
75 |
80 |
10 |
1): Absolute difference in biodegradation between bottles A and B |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'overall remarks' for details on validity criteria
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item was 80% biodegraded after 28 days, and reached at least 60% within a 10-day window. Therefore, the test item is considered readily biodegradable under the conditions of the Manometric Respirometry Test.
- Executive summary:
In a Manometric Respirometry Test, according to OECD guideline 301 F and GLP principles, the test substance was assessed for its ready biodegradability. The test substance was tested in duplicate at a target concentration of 31 mg/L, corresponding to a ThOD of 80 mg O2/L. The exposure period was 28 days and two inoculum blanks, a positive control and a toxicity control were included. The test item was added directly into the test bottles. The test solutions were continuously stirred during the test to ensure optimal contact between the test item and test organisms. The test substance was 75% and 85% biodegraded, in the duplicate bottles measured. Furthermore, at least 60% biodegradation was reached within a 10 -day window. Based on the average biodegradation of 80%, the substance is classified as readily biodegradable in the Manometric Respirometry Test. The test substance was found not to inhibit microbial activity and all validity criteria were met, thus the study was considered to be valid and reliable without restriction.
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