3-(methylthio)propionaldehyde

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002-05-21 - 2002-06-13

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: All test concentrations at 0 h. For stability control a series of the concentrations tested, was made up in the same way as the concentrations for biological part, but in separate vessels without test organisms and NaHCO3.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by adding 102 mg of the test item to 1 L synthetic freshwater without NaHCO3. For the biological part of the test, the stock solution was supplied with NaHCO3, to achieve the composition of synthetic water.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: waterflea
- Strain/clone: clone 5
- Age at study initiation (mean and range, SD): >24 h
- Source: originally obtained from Bayer AG, Leverkusen, Germany and bred in house
- Method of breeding: bred in M4 medium, fed daily with Desmodesmus subspicatus ad libitum.
- Feeding during test : no

ACCLIMATION
- Acclimation period: same as test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

250 mg/L (CaCO3)

Test temperature:

20 °C ± 1 °C

pH:

7.6 - 8.2

Dissolved oxygen:

7.4 - 8.5 mg/L

Nominal and measured concentrations:

Nominal test concentrations: 0.15, 0.3, 0.6, 1.2, 2.5, 5, 10 mg/L

Stability control:
DOC analysis 0 h: < LOQ, < LOQ, < LOQ, < LOQ, 2.86, 5.76, 11.25 mg/L
DOC analysis 48 h:< LOQ, < LOQ, < LOQ, < LOQ, < LOQ, 4.77, 10.31 mg/L

Test concentrations:
DOC analysis 0 h: < LOQ, < LOQ, < LOQ, < LOQ, 2.8, 6.2, 12.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 22 mL head-space test tubes
- Fill volume: 20 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater
- Ca/mg ratio: 4:1

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 h

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

4.5 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: confidence limits: 2.4 - 8.3 mg/L

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 12.4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

1.72 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

Reference item is tested quarterly:
1 mg K2 Cr2 O7 / L <= 50 % effect (<= EC50)
2 mg K2 Cr2 O7 / L >= 50 % effect ( >= EC50)

Reported statistics and error estimates:

The EC50 (48 h) was calculated by probit analysis according to Cavalli-Sforza (1972).

 Table 1: Biological effects

Concentration (mg/L)	number	Number mobile	Number immobile	%
Control	20	20	0	0
0.15	20	20	0	0
0.3	20	20	0	0
0.6	20	20	0	0
1.2	20	20	0	0
2.8	20	20	0	0
6.2	20	15	5	25
12.4	20	12	8	40

 

 

Validity criteria fulfilled:

yes

Conclusions:

An EC50 (48 h) of 4.5 mg/L based on measured concentrations (TOC analysis) was determined.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1985

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

according to guideline

Guideline:

other: AFNOR T 90 301

Version / remarks:

1983

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Nominal and measured concentrations:

not indicated

Details on test conditions:

ratio Ca/Mg 4:1
conductivity equal to 250 mg/L ± 25 mg/L

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

not specified

Conclusions:

The determined EC50 (24 h) was 25 mg/L.

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

GLP compliance:

not specified

Analytical monitoring:

yes

Details on sampling:

- Sampling method: At the beginning of exposure, before water change

Test organisms (species):

Daphnia magna

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 ± 1 °C

Nominal and measured concentrations:

Nominal test substance concentrations: 2.96, 4.44, 6.67, 10.0, 15.0 mg/L

Details on test conditions:

TEST SYSTEM
- Fill volume: 200 mL
- Renewal rate of test solution (frequency): every 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark
- Light intensity: indoor light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mobility after 24 and 48 h

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.15 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence limit: 6.30-8.06 mg/L

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

4.44 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Description of key information

EC50 (48 h): 4.5 mg/L (meas. TOC, OECD 202)

Additional information

The acute toxicity of the substance to Daphnia magna was investigated under static conditions in a guideline study according to OECD 202 (GLP). The test animals exposed to nominal test substance concentrations of 0.15, 0.3, 0.6, 1.2, 2.5, 5, 10, 100 mg/L for 48 h. An EC50 (48 h) of 4.5 mg/L (meas. initial based on TOC) was determined. A comparable result was obtained in a supporting study conducted according to OECD 202. Daphnia magna were exposed for 48 h to nominal test substance concentrations of 2.96, 4.44, 6.67, 10.0 and 15.0 mg/L. The test solutions were re-newed every 24 h and an analytical dose verfication by HPLC was performed. The determined EC50 (48 h) was 7.15 mg/L.

Supporting information is available from a study conducted according to AFNOR standard T 90 301N. In the study an EC50 (24 h) of 25 mg/L (nominal) was determined. However, since the test duration was only 24 h and the documentation of the study is not sufficient this result is not used for the assessment.