Tetrasodium 7,7'-[[6-(morpholin-4-yl)-1,3,5-triazine-2,4-diyl]diimino]bis[4-hydroxy-3-[(4-methoxy-2-sulphonatophenyl)azo]naphthalene-2-sulphonate]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015-04-27~2015-04-29

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test organisms (species):

Daphnia magna

Water media type:

freshwater

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Table 1 : Pre-test for the immobility and observed abnormal response of Daphnia magna immobilization test

Group	Immobility ( piece )		Observed abnormal response ( piece )
	24hr	48hr	24hr	48hr
Control	0	0	—	—
Test group (mg/L)
100	0	0	0	0
50	0	0	0	0
25	0	0	0	0
12.5	0	0	0	0
6.25	0	0	0	0
3.125	0	0	0	0
1.5625	0	0	0	0

Table 2 : The changes of pH values and oxygen concentration of Daphnia magna acute immobilization test

Group	pH values			Oxygen concentration (mg/L)
	0hr	48hr	△pH values	0hr	48hr
Control	9.00 ± 0.01	8.36 ± 0.03	-0.64	8.03 ± 0.10	7.98 ± 0.11
Test group (mg/L)
100	7.72 ± 0.09	7.47 ± 0.04	-0.25	7.76 ± 0.27	7.59 ± 0.27
50	7.96 ± 0.06	7.55 ± 0.07	-0.41	8.03 ± 0.09	7.81 ± 0.24
25	8.23 ± 0.03	7.60 ± 0.06	-0.63	7.71 ± 0.24	7.57 ± 0.23
12.5	8.55 ± 0.03	7.90 ± 0.08	-0.65	7.81 ± 0.15	7.67 ± 0.07
6.25	8.77 ± 0.02	8.11 ± 0.02	-0.66	7.75 ± 0.27	7.59 ± 0.24

Table 3 : The piece and percentage of observed abnormal response of Daphnia magna acute immobilization test

Group	Observed abnormal response ( piece )		Observed abnormal response ( % )
	24hr	48hr	24hr	48hr
Control	—	—	0	0
Test group (mg/L)
100	0	1	0	5
50	0	0	0	0
25	0	0	0	0
12.5	0	0	0	0
6.25	0	0	0	0

Table 4 : The piece and percentage of immobility of Daphnia magna acute immobilization test

Group	Immobility ( piece )		Immobility ( % )
	24hr	48hr	24hr	48hr
Control	—	—	0	0
Test group (mg/L)
100	0	1	0	5
50	0	0	0	0
25	0	0	0	0
12.5	0	0	0	0
6.25	0	0	0	0

Validity criteria fulfilled:

yes

Conclusions:

After microscopic observation of the growth of daphnid for 48hrs (qualitative analysis), we calculated the percentage of immobilization of Daphina magna activity at 0, 24, and 48 hr (quantitative analysis). The percentage of immobility of Daphnia magna for 48hrs treated concentration of the test substance, plotting the dose-response curve. And by linear interpolation calculation, EC50 of the test substance was greater than 100 mg/L.

Executive summary:

According to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test), EC50 of the test substance was greater than 100 mg/L.

Description of key information

EC50 of the test substance was greater than 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

In pre-test, Daphnia magna that were treated with the concentration of test substance of 100 mg/L with acute immobilization. Thus, the concentration of test substance was 100 mg/L for the highest dosage. The △pH values of control and test groups was less than ±1.5, and the concentration of dissolved oxygen at the end of the test was more than 3 mg/L in control and test groups. The results of this test came up to quality requirements, so that the data in this test were valid. According to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test), EC50 of the test substance was greater than 100 mg/L.