3-oxopregn-4-ene-21,17α-carbolactone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Study period:

09. Oct to 08. Dec 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well reported, guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-oxopregn-4-ene-21,17α-carbolactone

EC Number:

213-552-4

EC Name:

3-oxopregn-4-ene-21,17α-carbolactone

Cas Number:

976-70-5

Molecular formula:

C22H30O3

IUPAC Name:

(1R,3aS,3bR,9aR,9bS,11aS)-9a,11a-dimethyl-2,3,3a,3b,4,5,7,8,9,9a,9b,10,11,11a-tetradecahydrospiro[cyclopenta[a]phenanthrene-1,2'-oxolane]-5',7-dione

Details on test material:

- Name of test material (as cited in study report): Aldona (ZK 57564)
- Analytical purity: 95.5%
- Lot/batch No.: 59035042
- Expiration date of the lot/batch: Aug 2002

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 16.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48

Dose descriptor:

LOEC

Effect conc.:

ca. 29.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 26.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 29.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The no-observable effect-concentration (NOEC) of aldona on mobility (48 h) is 16.6 mg/l

Executive summary:

The aquatic toxicity was studied in an acute immobilization test with Daphnia magnaa ccording to test guideline OECD 202. The daphnids were exposed over 48 hours to 5 concentrations of the test substance between 2.8 and 29.8 mg/L, measured by TOC analysis and a control. There was a concentration related immobilization. The EC50/24h of aldona was higher than 29.8 mg/L, the EC50/48 hours was determined with 26.9 mg/L. The NOEC/48h was 16.6 mg/L, the LOEC/48h was 29.8 mg/L.