Trisodium 3-hydroxy-4-(4'-sulphonatonaphthylazo)naphthalene-2,7-disulphonate

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: data from peer - reviewed journals

Data source

Reference

Reference Type:

publication

Title:

STUDIES ON THE TOXICITY OF COAL-TAR DYES II. EXAMINATION OF THE BIOLOGICAL REACTION OF COAL-TAR DYES TO VITAL BODY

Author:

Yasuhide Tonogai, Yoshio Ito, Masahiro Iwaida, Masatomo Tati, Youki Ose and Takahiko Sato

Year:

1979

Bibliographic source:

The Journal of Toxicological Sciences , Vol 14. 211.-220, 1979

Materials and methods

Principles of method if other than guideline:

The toxicity of Amaranth was studied by various interactions between the dye and the components in vital body.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Trisodium 3-hydroxy-4-(4'-sulphonatonaphthylazo)naphthalene-2,7-disulphonate

EC Number:

213-022-2

EC Name:

Trisodium 3-hydroxy-4-(4'-sulphonatonaphthylazo)naphthalene-2,7-disulphonate

Cas Number:

915-67-3

Molecular formula:

C20H14N2O10S3.3Na

IUPAC Name:

trisodium 3-hydroxy-4-[(4-sulfonato-1-naphthyl)diazenyl]naphthalene-2,7-disulfonate

Constituent 2

Reference substance name:

2,7-Naphthalenedisulfonic acid, 3- hydroxy-4-[2-(4-sulfo-1-naphthalenyl)diazenyl]-, sodium salt (1 : 3)

IUPAC Name:

2,7-Naphthalenedisulfonic acid, 3- hydroxy-4-[2-(4-sulfo-1-naphthalenyl)diazenyl]-, sodium salt (1 : 3)

Constituent 3

Reference substance name:

Amaranth dye

IUPAC Name:

Amaranth dye

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Name of test material (as cited in study report): Amaranth dye
Molecular formula (if other than submission substance): C20H11N2Na3O10S3
Molecular weight (if other than submission substance): 604.47
Smiles notation (if other than submission substance): c1ccc2c(c1)c(ccc2S(=O)(=O)[O-])N=Nc3c4ccc(cc4cc(c3O)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+]
InChl (if other than submission substance): 1S/C20H14N2O10S3.3Na/c23-20-18(35(30,31)32)10-11-9-12(33(24,25)26)5-6-13(11)19(20)22-21-16-7-8-17(34(27,28)29)15-4-2-1-3-14(15)16;;;/h1-10,23H,(H,24,25,26)(H,27,28,29)(H,30,31,32);;;/q;3*+1/p-3
Substance type: Organic
Physical state: Solid

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no data

Radiolabelling:

no

Sampling and analysis

Details on sampling:

no data

Test solutions

Vehicle:

no

Details on preparation of test solutions, spiked fish food or sediment:

no data

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
Common name: Carp
Strain: no
Source: no data
Age at study initiation (mean and range, SD): same age
Length at study initiation (lenght definition, mean, range and SD): about 10cm in length
Weight at study initiation (mean and range, SD): 300 g in weight

ACCLIMATION
Acclimation period:10 days
Acclimation conditions (same as test or not): the fishes were acclimated for 10 days
Type and amount of food: No data
Feeding frequency: No data
Health during acclimation (any mortality observed): No data

Study design

Route of exposure:

aqueous

Test type:

static

Water / sediment media type:

not specified

Remarks:

freshwater

Total exposure / uptake duration:

180 min

Test conditions

Hardness:

no data

Test temperature:

no data

pH:

no data

Dissolved oxygen:

no data

TOC:

no data

Salinity:

no data

Details on test conditions:

no data

Nominal and measured concentrations:

nominal concentrations

Reference substance (positive control):

not specified

Details on estimation of bioconcentration:

BASIS INFORMATION
The amount of adsorbed dye on the gill of fish was measured spectrophotometrically

Results and discussion

Bioaccumulation factor

Conc. / dose:

100 mg/L

Type:

BCF

Remarks on result:

other: Test chemical was not adsorbed on the gills and intestines of fishes

Details on results:

The Test chemical was not adsorbed on the gills and intestines of fishes

Reported statistics:

no data

Any other information on results incl. tables

Table 1: dye adsorbed on gill and intestine in fish

 

Organ	Time (minutes)	Amount of dye adsorbed (µg/g)
Gill	30	0
	60	0
	180	2.0
Intestines	30	0
	60	0
	180	0

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The Test chemical was not adsorbed on the gills and intestines of fishes.
Based on these results, we can conclude that the test chemical is not bioaccumulative.

Executive summary:

The toxicity of Amaranth was studied by various interaction between the dye and the components in vital body.

Carp of same age, length 10cm and weight approx 300g were used. They were acclimated for 10 days in tap water before experiment.

 

Fish were exposed to Amaranth solution (100mg/l)for 30, 60 and 180 min and then gills and intestine were taken out by dissection and the adsorbed dye was eluted with 70% ethanol containing 1% ammonia water, determined spectrophotometrically and expressed as amount of adsorbed dye on each organ.

The Test chemical was not adsorbed on the gills and intestines of fishes.

Based on these results, we can conclude that the test chemical is not bioaccumulative.