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EC number: 212-811-9 | CAS number: 871-78-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-06-09
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Principles of method if other than guideline:
- In contrast to the guideline, it was decided to use copper (II) suphate pentahydrate instead of the prescribed 3,5-dichlorophenol. The usage of dichlorophenol is unsuitable for routine lab use.
- GLP compliance:
- yes
Test material
- Reference substance name:
- N,N'-ethylenedi(diacetamide)
- EC Number:
- 212-811-9
- EC Name:
- N,N'-ethylenedi(diacetamide)
- Cas Number:
- 871-78-3
- Molecular formula:
- C6H12N2O2
- IUPAC Name:
- N-(2-acetamidoethyl)acetamide
- Reference substance name:
- Diacetylethylenediamine (DAED)
- IUPAC Name:
- Diacetylethylenediamine (DAED)
- Details on test material:
- - Name of test material (as cited in study report): N,N'-Diacetylethylenediamine (DAED)
- Physical state: Solid, powder
- Analytical purity: 99.9 %
- Lot/batch No.: 14-016
- Expiration date of the lot/batch: 2010-05-21
- Storage condition of test material: Room temperature, protected from moisture and light
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1000 mg/L were weighed out, dispersion treatment was agitation
- Differential loading: 1000 mg/L
- Controls: 2 control samples without test substance were included
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure: Non adapted activated sludge from the sewage plant at sarstedt (Municipal sewage treatment plant of 31157 Sarstedt, Germany)
- Pretreatment: Washed twice with autoclaved tap water and adjusted to a dry weight concentration of 4.0 g/L +/- 10 %
- Dry sludge concentration: 4.38 g/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- Not determined
- Test temperature:
- 20.4 °C
- pH:
- 6.64
- Salinity:
- Not determined
- Nominal and measured concentrations:
- 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL Erlenmeyer flask, DIN 12380
- Aeration: Permanent
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Composition of test medium: 200 mL inoculum + 16 mL synthetic waste water (acc. to the guideline) + 284 mL autoclaved tap water + test substance concentration (directly weighing of 500 mg)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Determination of respiration rates subsequently to an incubation period of 3 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: None, limit test concentration
- Range finding study
- Test concentrations: 10 - 100 - 1000 mg/L
- Results used to determine the conditions for the definitive study: Yes
Inhibitions of the Range-Finding Test
Concentration
[mg/L] Inhibition
[%]
10 3
100 1
1000 2 - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50: 109 (CI 54 - 156) mg/L - Reported statistics and error estimates:
- The EC50 of the reference item was calculated with probit analysis using software SIGMAPLOT (Windows), SPSS CORPORATION.
Probit values were taken from WEBER (1986).
Any other information on results incl. tables
From the oxygen depletion the respiration rates were calculated in mg O2/L×h (Table 2).
The inhibition of the respiration rates from the test and reference item concentrations in % was calculated in comparison with the respiration rates from the control.
Respiration Rates and Inhibition of Respiration of Test and Reference Item Concentrations
Concentration |
Respiration rate |
Inhibition |
|
[mg/L] |
[mgO2/L×h] |
[%] |
|
Test item |
1000 |
29.2 |
-1 |
Control 1 |
– |
28.4 |
– |
Control 2 |
– |
29.2 |
– |
Reference item |
180 |
8.4 |
71 |
100 |
14.8 |
49 |
|
58 |
22.4 |
22 |
Difference of the control respiration rates: 3 %
Promotion of respiration is signed with minus (-)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item N,N’-Diacetylethylenediamine (DAED) is not toxic at concentrations
> 1000 mg/L to activated sludge of a municipal sewage treatment plant. - Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item N,N’-Diacetylethylenediamine (DAED) (batch-no. 14-016) on 2009-06-09 at Dr.U.Noack-Laboratorien, D-31157 Sarstedt. Test system was activated sludge of the municipal treatment plant of D-31157 Sarstedt. The test was carried out under static conditions with the limit concentration 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. No inhibition was observed for the test item.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper-(II)-sulphate-pentahydrate as reference item and the reference toxicity was determined. The EC50- value for the reference item was 109 mg/L.
Validity criteria of the test guideline were fulfilled.
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