Sodium 2-amino-4,6-dinitrophenoxide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: estimated by calculation

Justification for type of information:

estimated by calculation

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

Estimation of 96 hr EC50 value of test chemical by modelling Database - ECOSAR v.1.11. The ECOSAR Class Program is a computerized version of the ECOSAR analysis procedures as currently practiced by the Office of Pollution Prevention and Toxics (OPPT).  It has been developed within the regulatory constraints of the Toxic Substances Control Act (TSCA).  It is a pragmatic approach to SAR. The structure-activity relationships (SARs) presented in this program are used to predict the aquatic toxicity of chemicals based on their similarity of structure to chemicals for which the aquatic toxicity has been previously measured.  Most SAR calculations in the ECOSAR Class Program are based upon the octanol/water partition coefficient (Kow).  Various surfactant SAR calculations are based upon the average length of carbon chains or the number of ethoxylate units.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Test type:

not specified

Water media type:

not specified

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

1 637.454 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: not specified

Validity criteria fulfilled:

not specified

Conclusions:

The Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 1637.454 mg/L.

Executive summary:

The Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 1637.454 mg/L. Thus considering the value from CLP Criteria for aquatic classification of the substance , it is concluded that test material does not exhibit short term toxicity aquatic invertebrate (Daphnia Magna) in the present test condition.

Description of key information

Short term toxicity to aquatic invertebrates:

The Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 1637.454 mg/L. Thus considering the value from CLP Criteria for aquatic classification of the substance , it is concluded that test material does not exhibit short term toxicity aquatic invertebrate (Daphnia Magna) in the present test condition.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 637.454 mg/L

Additional information

Short term toxicity to aquatic invertebrates:

The toxicity of test material was evaluated for aquatic invertebrate based on the predicted data and data for structurally similar read across substance from peer reviewed journal.

The Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 1637.454 mg/L. Thus considering the value from CLP Criteria for aquatic classification of the substance , it is concluded that test material does not exhibit short term toxicity aquatic invertebrate (Daphnia Magna) in the present test condition.

The above prediction was supported by data from peer reviewed journal for structurally similar read across substance ,Short term toxicity of test material was evaluated on aquatic invertebrate , for 24 h .The dilution water used had a specific composition: acid capacity Ks4.3 of 0.8mmoll -I total hardness of 2.4 mmol/l , a calcium to magnesium ratio of 4:1, a sodium to potassium ratio of 10:1 and thus a resulting initial pH value of 8.0 + 0.2. 6-24 h old daphnids were used , test was carried out in 50 ml beakers with ground-glass stoppers each with 20 ml useful capacity. The effect concentration noted after 24 h was observed to be 123 mg/l. Based on the above effect concentration it can be considered that test material is not toxic to daphnia magna and hence, can not be classified as per CLP criteria.

Another study , for structurally similar read across substance stated that toxicity of test material was evlauted on American Oysters . Clutchless American oysters (x = 48.4 ± 6.2 mm shell height) were purchased from the Wilde Oyster Hatchery, Shady Side, Maryland.test organisms were obtained at approximately 10 C and acclimated in the laboratory for a minimum of two weeks at 10 (+ 1) C under a 12 hlight:12 h dark photoperiod prior to and during testing. Oysters were fed natural plankton in the estuarine water system supplemented with 1.5 percent corn starch-yeast solution when necessary.One replicate of 20 organisms was used at each test concentration , the concentrations used were 0, 25, 50, 100, 150, and 200 mg/l. The effect of test material was on organism American Oysters for 144 h , was observed to be 254.9 mg/l.

Based on the above effect concentration it can be concluded that test material is not toxic to aquatic invertebrates.