Sodium 2-amino-4,6-dinitrophenoxide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from computational model developed by USEPA

Justification for type of information:

Data is from computational model developed by USEPA

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

ECOSAR v1.00

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: Fish

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

no data

Test temperature:

no data

pH:

no data

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

no data

Details on test conditions:

no data

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1 920.657 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: ECOSAR Class: Neutral Organic SAR(Baseline Toxicity)

Conclusions:

Based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10, the 96 hours LC50 was estimated to be 1920.657 mg/l in freshwater on fish for substance with mortality effects.

Executive summary:

Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to fish was predicted. On the basis of this program, the LC50 value for short term toxicity to aquatic invertebrates was predicted to be 1920.657 mg/l for test material in 48 hrs. Based on the above effect concentration value it can be predicted test substance is not toxic to fish and can not be classified as per CLP criteria.

Description of key information

Short term toxicity to fish:

Based on the p iction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to fish was predicted. On the basis of this program, the LC50 value for short term toxicity to aquatic invertebrates was predicted to be1920.657mg/l for test material in 48 hrs. Based on the above effect concentration value it can be predicted test substance is not toxic to fish and can not be classified as per CLP criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 920.657 mg/L

Additional information

Short term toxicity to fish:

The toxicity of test material was evaluated for fish based on the predicted data and data for structurally similar read across substance from peer reviewed journal

Based on the piction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to fish was predicted. On the basis of this program, the LC50 value for short term toxicity to aquatic invertebrates was predicted to be1920.657mg/l for test material in 48 hrs. Based on the above effect concentration value it can be predicted test substance is not toxic to fish and can not be classified as per CLP criteria.

The short term toxicity of test material on fish Oncorhynchus mykiss (Rainbow trout) was observed for 96 h Juvenile rainbow trout (x = 41.6±8.7 mm total length) were selected .Rainbow trout were held in the laboratory at an approximate density o 3 g/l in continuous flow, non - chlorinated deep well water with 99 percent replacement of the water every 12 h. The fish were acclimatized for 2 weeks in the same conditions as test ,during acclimation, the trout were fed commercial trout chow daily, static renewations were conducted. In an effort to control possible volatilization, the aquaria were fitted with glass covers. Test chambers were placed in two 4 x 0.8 x 0.25 m circulated constant temperature baths. The dilution water used during the tests was the same as that used for acclimation.Two replicates composed of a total of 25 fish (N = 13 and 12) were randomly selected from the test population and placed in the aquaria containing the test solutions. The nominal test concentrations for test material were 0, 75, 85, 95, 105, 115, and 125 mg/l. The lethal effect concentration (LC50) observed after 96 h was noted to be 135.0 mg/l. As no mortalities were observed till the concentration 135.0 mg/l , it can be concluded that the test material is not classified as per CLP criteria.

For another structurally similar read across substance, the short term toxicity of test material on fish Oncorhynchus mykiss (Rainbow trout) was observed for 96 h Juvenile rainbow trout (x = 41.6±8.7 mm total length) were selected .Rainbow trout were held in the laboratory at an approximate density o 3 g/l in continuous flow, non - chlorinated deep well water with 99 percent replacement of the water every 12 h. The fish were acclimatized for 2 weeks in the same conditions as test ,during acclimation, the trout were fed commercial trout chow daily, static renewations were conducted. In an effort to control possible volatilization, the aquaria were fitted with glass covers. Test chambers were placed in two 4 x 0.8 x 0.25 m circulated constant temperature baths. The dilution water used during the tests was the same as that used for acclimation.Two replicates composed of a total of 25 fish (N = 13 and 12) were randomly selected from the test population and placed in the aquaria containing the test solutions. The nominal test concentrations for test material were 0, 75, 85, 95, 105, 115, and 125 mg/l. The lethal effect concentration (LC50) observed after 96 h was noted to be 135.0 mg/l.

As no mortalities were observed till the concentration 219.2 mg/l , it can be concluded that the test material is not classified as per CLP criteria.