2,2,6,6-tetramethyl-4-piperidone

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test)

Version / remarks:

Cited as Directive 84/449/EEC, C.3

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2,6,6-tetramethyl-4-piperidone

EC Number:

212-554-2

EC Name:

2,2,6,6-tetramethyl-4-piperidone

Cas Number:

826-36-8

Molecular formula:

C9H17NO

IUPAC Name:

2,2,6,6-tetramethylpiperidin-4-one

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): municipal STP, Marl-Ost
- Water filtered: yes

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

11.6 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: 2 mL mineral nutrient solution, 2 mL micronutrients solution, 2 mL vitamin solution, 1 mL inoculum, test item solution resulting in approx. 10 mg DOC/L, water added up to a volume of 2 L
- Test temperature: 25-25.1°C
- pH adjusted: no data
- Continuous darkness: yes

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Number of inoculum blank samples: 2
- Number of reference substance samples: 2

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

11

Sampling time:

28 d

Details on results:

Kinetic of test substance (in % DOC reduction):
7% after 7 day(s)
11% after 14 day(s)
4% after 21 day(s)
7% after 27 day(s)
11% after 28 day(s)

BOD5 / COD results

Results with reference substance:

Kinetic of control substance (in % DOC reduction):
98% after 14 day(s)
100% after 28 day(s)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

In this screening test for ready degradability the test item triacetonamine showed a degradation of 11% after 28 days.