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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
other information
Study period:
19.06.1998
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1998
Report date:
1998

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Methoxyacetic acid
EC Number:
210-894-6
EC Name:
Methoxyacetic acid
Cas Number:
625-45-6
Molecular formula:
C3H6O3
IUPAC Name:
2-methoxyacetic acid
Constituent 2
Reference substance name:
2-methoxyacetic acid
IUPAC Name:
2-methoxyacetic acid
Details on test material:
Batch: 351681/1796 (deliverd by Fluka)
water solubility: mixable in any relation till 10 g/L
purity: 98 %
Homogeneity: homogeneous
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data available

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
no data available

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
Activated sludge from laboratory wastewater treatment plants fed with municipal and synthetic wastewater. The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated for 24 hours. 50 ml were added to a test volume of 250 ml to obtain a concentration of 1 g/L dry substance in the test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
30 min
Post exposure observation period:
no data available

Test conditions

Hardness:
no data available
Test temperature:
20 +/- 2 °C
pH:
no data available
Dissolved oxygen:
> 2.5 mg/L during aeration
>6.5 mg/L immidiately before measurement
Salinity:
no data available
Nominal and measured concentrations:
nominal concentration of 1000 mg/L
Details on test conditions:
Stock solution
Name input (g)
Test substance Methoxyessigsäure 2 g/L
Reference substance 3,5-dichlorophenol 0.5 g/L

Synthetic medium: 8 mI/vessel 100-fold concentrated OECD Medium
The pH value has been measured and adjusted to pH 7.0 - 8.0 before the addition of activated sludge.
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
The IC50 for 3,5-dichlorophenol was ca. 12 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on the results of this study the IC50 of Methoxyacetic acid on activated sludge is > 1000 mg/L.
Executive summary:

This study was designed to determine the effects of Methoxyacetic acid on sewage sludge micro-organisms by measuring the respiration rate after exposure to the test substance after a 30 min contact time. Based on the results of this study the EC50 of Methoxyacetic on activated sludge is higher than 1000 mg/L.