4-ethoxybenzoic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from publication

Justification for type of information:

Data is from United States Department of the Interior, Fred A. Seaton, Secretary Fish and Wildlife Service.

Qualifier:

according to guideline

Guideline:

other: As per mention below

Version / remarks:

No data available

Deviations:

not specified

Principles of method if other than guideline:

Short term toxicity study of 4-Ethoxybenzoic acid on the growth of fishes Petromyzon marinus, Salmo gairdnerii and Lepomis macrochinus for 24 hrs.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): Benzoic acid, 4-ethoxy-
- Molecular formula (if other than submission substance): C9H10O3
- Molecular weight (if other than submission substance): 166.175g/mol
- Smiles notation (if other than submission substance): c1(ccc(OCC)cc1)C(O)=O
- InChl (if other than submission substance): 1S/C9H10O3/c1-2-12-8-5-3-7(4-6-8)9(10)11/h3-6H,2H2,1H3,(H,10,11)
- Substance type: Organic
- Physical state: Solid
- Analytical purity: pure

Analytical monitoring:

not specified

Details on sampling:

- Concentrations: 5 mg/l (5ppm)
- Sampling method: No data available
- Sample storage conditions before analysis: specimens were maintained in the best possible physical condition until used in the laboratory.

Vehicle:

yes

Remarks:

acetone or alcohol was used as a solvent

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 5 mg/l Chemicals were weighed in calibrated weighing bottles to the nearest milligram on a Volland Speedigram balance. Each concentrated mixture was next added to a predetermined volume of water (as required by actual weight of Sample and desired concentration) and agitated with a Power-Stir to produce a more dilute solution.
Emulsions or suspensions of insoluble compounds were made with the aid of a Waring blender. These prepared solutions, emulsions, or suspensions were added to the test containers in which the experimental animals had already been placed.

- Eluate: No data available
- Differential loading: No data available
- Controls: No data available
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone or alcohol
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): Five cubic centimeters of the indicated solvent was added to each sample.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No data available

Test organisms (species):

other: Petromyzon marinus, Salmo gairdnerii and Lepomis macrochirus

Details on test organisms:

TEST ORGANISM
- Common name: Sea lamprey for Petromyzon marinus, Rainbow trout for Salmo gairdnerii and Bluegill sunfish for Lepomis macrochinus
- Source: Test larva and fishes were collected by means of an electric shocker in the Ocqueo River, Presque Isle County, Michigan, and were held in running water in aquaria and small "races" under conditions which stimulated their natural stream habitat. Stock of local state and federal fish hatcheries.
- Age at study initiation (mean and range, SD): Fingerling
- Length at study initiation (length definition, mean, range and SD): 3 to 5 inches in total length.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

24 h

Remarks on exposure duration:

No data available

Post exposure observation period:

No data available

Hardness:

No data available

Test temperature:

55° C

pH:

7.5 to 8.2

Dissolved oxygen:

8.6 to 13.7 ppm

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

Measured concentration 5 mg/l Chemicals were weighed in calibrated weighing bottles to the nearest milligram on a Volland Speedigram balance.

Details on test conditions:

- Test vessel: Glass battery jar
- Material, size, headspace, fill volume: 10 liter of glass battery jar contain 5 liter of water having the headspace of 5 liter.
- Aeration: These jars were provided with aeration through standard stone air - breakers (al near oxygen saturation, as determined by repeated tests) and were maintained at a constant temp by Immersion in specially constructed constant temperature troughs.
- No. of organisms per vessel: 6
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Other: Twelve of test jars (each containing a substance being assayed)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Water used in all tests was drawn from a supply pumped directly from Hammond Bay of Lake Huron. The suction line intake of this pumping system was located 250 feet offshore at 3 depths of about 9 feet. Water from this source was consistently clear and of a relatively uniform quality
- Total organic carbon: 5 to 9 p. p. m.

Reference substance (positive control):

not specified

Key result

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No effect was obtain

Details on results:

- Behavioural abnormalities: No effect was observed.

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

No data available

Sublethal observations / clinical signs:

Any chemical killing the larval lampreys in eight hours or less at a concentration of 5.0 p. p. m.(regardless of the effects on other fishes) was tested further at levels of 1.0 and 0.1 p. p. m. Water temperature, test period, and procedures were identical with those described for the initial test at 5mg/l.

Validity criteria fulfilled:

not specified

Conclusions:

Based on the no effect and mortality of fishes Petromyzon marinus, Salmo gairdnerii and Lepomis macrochirus, due to the Benzoic acid, 4-ethoxy-, the EC0 was 5 ppm.

Executive summary:

Short term toxicity study of Benzoic acid, 4-ethoxy- on the growth of fishes Petromyzon marinus, Salmo gairdnerii and Lepomis macrochirus for 24 h. Measured concentration of 5 mg/l Chemicals were weighed in calibrated weighing bottles to the nearest milligram on a Volland Speedigram balance. Test performed in the 10 liter of glass battery jar contain 5 liter of water having the headspace of 5 liter. After the exposure of 24 hrs with chemical no any mortality and other effect was observed.

 

Based on the no effect and mortality of fishes Petromyzon marinus, Salmo gairdnerii and Lepomis macrochirus, due to the Benzoic acid, 4-ethoxy-, the EC0 was 5 ppm.

Description of key information

Short term toxicity study of Benzoic acid, 4-ethoxy- on the growth of fishes Petromyzon marinus, Salmo gairdnerii and Lepomis macrochirus for 24 h Measured concentration of 5 mg/l Chemicals were weighed in calibrated weighing bottles to the nearest milligram on a Volland Speedigram balance. Test performed in the 10 liter of glass battery jar contain 5 liter of water having the headspace of 5 liter. After the exposure of 24 hrs with chemical no any mortality and other effect was observed.

 

Based on the no effect and mortality of fishes Petromyzon marinus, Salmo gairdnerii and Lepomis macrochirus, due to the Benzoic acid, 4-ethoxy-, the EC0 was 5 ppm.

Additional information

Short term toxicity study of Benzoic acid, 4-ethoxy- on the growth of fishes Petromyzon marinus, Salmo gairdnerii and Lepomis macrochirus for 24 h Measured concentration of 5 mg/l Chemicals were weighed in calibrated weighing bottles to the nearest milligram on a Volland Speedigram balance. Test performed in the 10 liter of glass battery jar contain 5 liter of water having the headspace of 5 liter. After the exposure of 24 hrs with chemical no any mortality and other effect was observed.

 

Based on the no effect and mortality of fishes Petromyzon marinus, Salmo gairdnerii and Lepomis macrochirus, due to the Benzoic acid, 4-ethoxy-, the EC0 was 5 ppm.