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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
28-08-2017 - 01-09-2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard test guidelines
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material : sodium 3-[(4-anilinophenyl)diazenyl]benzenesulfonate
- Molecular formula : C18H14N3O3S.Na
- Molecular weight: 375.383 g/mol
- Smiles notation : c1(Nc2ccccc2)ccc(\N=N\c2cc(ccc2)S(=O)(=O)[O-])cc1.[Na+]
- InChl : 1S/C18H15N3O3S.Na/c22-25(23,24)18-8-4-7-17(13-18)21-20-16-11-9-15(10-12-16)19-14-5-2-1-3-6-14;/h1-13,19H,(H,22,23,24);/q;+1/p-1/b21-20+;
- Substance type: Organic
- Physical state: Solid
Analytical monitoring:
not specified
Vehicle:
yes
Details on test solutions:
Preparation of the test Solutions : The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of potable water (passed through reverse osmosis system) with one hour continuous stirring for achieving test concentrations of 1.56mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
Animal species: Zebra fish(Danio rerio)
Animal breeder/ supplier Koyal aquarium,Nagpur.
Body length: average 1.8 cm
:Body weight: 0.662g (average)

The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
Duration of housing: 15 days
Photoperiod:16 hours light and 8 hours dark
Oxygen content: 7.7 (mg/l)
pH: 7.6
Water temperature: 24.1 °C
Mortality (%): 0
Diet: Standard brand feed, once daily

Acclimatization: Fishes, to be used in the study, are kept in the test water for seven days immediately before testing under following conditions:
Photoperiod: 16 hours light and 8 hours dark
Temperature range: 23.05 °C
pH range: 7.7
DO range:7.8 mg/l
Feeding: Daily once
Aeration: Continuous
Mortality (%): During last 48 hours = 0
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
152.5 mg of CaCO3
Test temperature:
23°C
pH:
7.2
Dissolved oxygen:
7.4 mg/L
Nominal and measured concentrations:
1.56mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L
Details on test conditions:
Photoperiod: 16 hours light and 8 hours dark
Test vessels: The test vessels used were all plastic aquaria having the water capacity of 5liters.
Volume of water: 2 liters
No. of animals per vessel: 8 fishes
Test temperature: 23 °C
Adaptation: Test conditions corresponded to housing conditions
Test dates Test started at : 21-08-2017
Test Exposure date: 28-08-2017
Date of experimental termination : 01-09-2017
Withdrawal of food: 1 day before and during exposure
Aeration: Aeration in test vessels was provided 1 day before the start of experiment
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Sublethal observations / clinical signs:

 

pH and oxygen content:

Nominal

Concentration

(mg/l)

Number of

fish

pH readings after

24 h

48 h

72 h

96 h

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

7.6

7.6

7.9

7.9

8.0

8.0

8.2

8.2

1.56

4

4

7.8

7.8

7.9

7.9

8.1

8.1

8.3

8.3

3.125

4

4

7.8

7.8

7.9

7.9

8.1

8.1

8.3

8.3

6.25

4

4

7.8

7.8

8.0

8.0

8.1

8.1

8.4

8.4

12.5

4

4

7.8

7.8

8.0

8.0

8.1

8.1

8.4

8.4

25

4

4

7.9

7.9

8.0

8.0

8.1

8.1

8.4

8.4

 

Nominal

Concentration

(mg/l)

Number of

fish

Oxygen content (mg/L) after

24 h

48 h

72 h

96 h

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

7.0

7.0

6.9

6.9

6.8

6.8

6.6

6.6

1.56

4

4

6.9

6.9

6.8

6.8

6.6

6.6

6.5

6.5

3.125

4

4

6.9

6.9

6.7

6.7

6.6

6.6

6.5

6.5

6.25

4

4

6.9

6.9

6.7

6.7

6.6

6.6

6.5

6.5

12.5

4

4

6.9

6.9

6.7

6.7

6.6

6.6

6.5

6.5

25

4

4

6.9

6.9

6.7

6.7

6.6

6.6

6.5

6.5

 

Test temperature:

Nominal

Concentration

(mg/l)

Number of

fish

Temperature (°C) after

24 h

48 h

72 h

96 h

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

23.5

23.5

23.5

23.5

23.8

23.8

23.4

23.4

1.56

4

4

23.5

23.5

23.5

23.5

23.8

23.8

23.4

23.4

3.125

4

4

23.5

23.5

23.5

23.5

23.8

23.8

23.4

23.4

6.25

4

4

23.5

23.5

23.5

23.5

23.8

23.8

23.4

23.4

12.5

4

4

23.5

23.5

23.5

23.5

23.8

23.8

23.4

23.4

25

4

4

23.5

23.5

23.5

23.5

23.8

23.8

23.4

23.4

 

 

Mortality and symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3 h

6 h

24 h

48 h

72 h

96 h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

1.56

4

4

-

-

-

-

-

-

-

-

-

-

-

-

3.125

4

4

-

-

-

-

 

-

-

-

-

-

-

-

6.25

4

4

-

-

-

-

-

-

-

-

-

-

-

-

12.5

4

4

-

-

-

-

-

-

-

-

-

-

-

-

25

4

4

-

-

-

-

-

-

-

-

1

1

2

2

 

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Symptom (cumulated) after

3 h

6 h

24 h

48 h

72 h

96 h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

1.56

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

3.125

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

6.25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

12.5

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS3M1

NS3M1

NS2M1

NS2M1

NS2

NS2

 

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

 

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for sodium 3-[(4-anilinophenyl)diazenyl]benzenesulfonate CAS No.587-98-4 on Zebra fish (Danio rerio) was determined to be 25 mg/L.
Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for sodium 3-[(4-anilinophenyl)diazenyl]benzenesulfonate. The nominal concentration selected for the experiment were 1.56mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L and Zebra fish (Danio rerio) were exposed to these concentration for 96 hours. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was found to be 25 mg/L.

Thus, it can be concluded that the Sodium 3-(p-anilinophenylazo)benzenesulphonate is toxic to fish and can be considered as "aquatic chronic 3" under CLP regulation.

Description of key information

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for sodium 3-[(4-anilinophenyl)diazenyl]benzenesulfonate CAS No.587-98-4 on Zebra fish (Danio rerio) was determined to be 25 mg/L.

This value indicates that the Sodium 3-(p-anilinophenylazo)benzenesulphonate is toxic to fish and can be considered as "aquatic chronic 3" under CLP regulation.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
25 mg/L

Additional information

Two experimental studies were reviewed for short term fish endpoint of test substance Sodium 3-(p-anilinophenylazo)benzenesulphonate (CAS 587 -98 -4) which are summarised as follows.

In a key study, Fish Acute Toxicity test was conducted according to OECD Guideline 203 for sodium 3-[(4-anilinophenyl)diazenyl]benzenesulfonate. The nominal concentration selected for the experiment were 1.56mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/Land Zebra fish(Danio rerio) were exposed to these concentration for 96 hours.The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was found to be 25 mg/L.

Supporting study obtained from abstract of Journal for Nature Conservation. 1 (2): 155-157, 1989 indicates that the Channa punctate were exposed to the Sodium 3-(p-anilinophenylazo)benzenesulphonate preferably for a period of 96 hours. 50 % Mortalities of the fish (LC50) were determined. On the basis of 50% mortality the LC50 value is determined to be 1.5 mg/l.

Thus, based on the available effect concentrations from key study which is 25 mg/l for target it can be concludedn that test substance Sodium 3-(p-anilinophenylazo)benzenesulphonate (CAS 587 -98 -4) was likely to be toxic to fish, hence it can be considered to be“aquatic chronic 3”as per the CLP regulation.