29H,31H-phthalocyanine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

non-GLP, no analytical monitoring

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): Heliogen Gruen 8730;
- Lot/batch No.: BW 32
- Source: BASF-Heliogen-Fabrik
- Analytical purity: 98 %

Analytical monitoring:

no

Vehicle:

yes

Remarks:

Tween 80

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution contains 500 mg/l test substance with 100 mg/l Tween 80. The stock solution contains 500 mg/l test substance with 100 mg/l Tween 80 as solvent. The stock solution was stirred for about 10 minutes at 20°C.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus
- Age at study initiation (mean and range, SD): 2-24 h
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Stage and instar at study initiation:
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Source: cultured at the laboratories of BASF since 1978, originally obtained by Institut National de Recherche Chimique Appliquie, France
- Age of parental stock (mean and range, SD):
- Feeding during test : no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.44 mmol/l

Test temperature:

18.5 °C

pH:

7.49 - 7.78

Dissolved oxygen:

Oxygen content (after 0 hours): 9.05 - 9.41 mg/l
Oxygen content (after 48 hours): 7.35 - 7.96 mg/l

Nominal and measured concentrations:

Nominal test concentrations:
control, 62.5, 125, 250 and 500 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Material, size, headspace, fill volume: glass, 10 ml
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 ml volume/animal

TEST MEDIUM / WATER PARAMETERS
- Ca/mg ratio: 4 to 1
- Conductivity: 550 - 650 µSiemens/com
- Intervals of water quality measurement: initially and after 48 hours (end of exposure)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h day and 8 h night
- Light intensity: about 5 µE (wave length: 400-700 nm)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilization visually after 0, 3, 6, 24 and 48 hours

VEHICLE CONTROL PERFORMED: yes

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

Due to the results of the test no statistical evaluation was performed.

Table 1. Mortality

Concentration [mg/L]	0 h	24 h	48 h
Control	20	20	20
Solvent Control	20	20	20
62.5	20	20	20
125	20	20	20
250	20	20	20
500	20	20	20

 

Table 2. Dissolved oxygen [mg/L]

Concentration [mg/L]	0 h	48 h
Control	9.43	7.93
Solvent Control	8.62	6.49
62.5	9.41	7.96
125	9.31	7.84
250	9.23	7.48
500	9.05	7.35

 

Table 3 pH values

Concentration [mg/L]	0 h	48 h
Control	7.68	7.73
Solvent Control	7.36	7.4
62.5	7.68	7.78
125	7.65	7.7
250	7.59	7.64
500	7.49	7.61

 

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

non-GLP study, no analytical monitoring

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

The test follows the EU Directive 79/831/EEC, Annex V, part C, which is comparable to the OECD guideline 202.

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): Heliogen Blau 6920,
Purity ca. 98 %

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
The substance was prepared with addition of Tween 80 and dilution water. The stock solution contains 500 mg/l of the test substance and 100 mg/l of the dispersant. The stock solution was stirred 10 minutes. Following this, serial dilutions of this test medium were prepared in order to obtain the desired test concentrations. Additionally a culture medium and solvent control was tested in parallel.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: Daphnia magna Straus
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age of test organisms: 2 - 24 hours
- Feed: Whilst changing water Brewer's yeast was given, also 1 x daily grown Green algae culture

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.70 ± 0.5 mmol/l

Test temperature:

20 ± 1°C

pH:

7.7 - 8.8

Dissolved oxygen:

> 2 mg/l

Conductivity:

550 - 650 microS/cm

Nominal and measured concentrations:

Nominal test concentrations: 0, 62.5, 125, 250, 500 mg/l. A control was tested in parallel.

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Material, size, headspace, fill volume: glass
- Volume of solution: 10 mL
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates): 5


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the test guideline DIN 38412, L 11
- Ca/mg ratio: 4 :1
- Intervals of water quality measurement: 0, 3, 6, 24, 48 h

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity: diffuse light (5 microeinstein/m*m*s) at 400 - 750 nm wave length

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility after 0, 24, 48 h

VEHICLE CONTROL PERFORMED: yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Immobilization of daphnia after 48 hours, %:

Nominal test concentration [mg/L]	Immobilization [%]
Solvent Control	5
Control	15
62.5	10
125	5
250	0
500	5

 

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

non-GLP study, no analytical monitoring

Qualifier:

according to guideline

Guideline:

other: EPA-660/3-75-009

Principles of method if other than guideline:

according to "Committee on Methods for Toxicity Tests with Aquatic Organisms. Methods for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians. EPA-660/3-75-009. page 61 "

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): Fastolux Green

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

For each test concentration the test substance was added into a 1 liter beaker containing dilution water. This solution was mixed and then decanted 200 ml into each of four 250 ml beakers.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation (mean and range, SD): < 20 h
- Method of breeding: from a laboratory stock culture
- Source: National Water Quality Laboratory in Duluth, Minnesota.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

100 mg/l (as CaCO3)

Test temperature:

18 ±1 °C

pH:

7.11 - 7.30

Dissolved oxygen:

8.7 - 9.1 mg/l

Conductivity:

110 µ mhos/cm

Nominal and measured concentrations:

Nominal test concentrations: Control, 56, 100, 180, 320, 560 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass bicker
- Material, size, headspace, fill volume: 250 mL
- Volume of solution: 200 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 40 mL per animal

TEST MEDIUM / WATER PARAMETERS
- Alkalinity: 27 mg/l (as CaCO3)
- Conductivity: 110 µ mhos/cm
- Intervals of water quality measurement: initial and at the end of exposure (48 h)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility, initial and every 24 h

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

153.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other:

Remarks:

95% confidence interval (127.5 - 185.1)

Table 1. Number of immobile daphnias (from 20)

Concentration [mg/L]	24 h	48 h
Control	0	0
56	0	2
100	1	4
180	3	9
320	16	20
560	20	20

 

Table 2. Dissolved oxygen [mg/L]

Concentration [mg/L]	0 h	48 h
Control	9.0	8.8
56	9.0	8.8
180	9.1	8.7
560	8.8	8.8

 

Table 3 pH values

Concentration [mg/L]	0 h	48 h
Control	7.48	7.51
56	7.18	7.25
180	7.14	7.28
560	7.11	7.30

 

Description of key information

The substance is not acutely toxic to aquatic invertebrates, with an LC50 of 154 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

154 mg/L

Additional information

All substances in this category share a similar structure with a molecular weight > 500 g/mol. Based on the high molecular weight and insolubility, the substances in this category will not be able to penetrate biological membranes. Thus, no systemic exposure is expected. This is confirmed by the environmental studies on aquatic invertebrates for two substances in this category: CAS 1328-53-6 and CAS 147-14-8. The environmental toxicity data can be used for read-across to all substances in this category.

In all studies, Daphnia magna was used as the test organism, and no analytical measurements were performed. Two studies on substance CAS 1328-53-6 are available. In the study conducted according to EPA-660/3-75-009, the 48-h EC50 for mobility was determined to be 154 mg/L (Sun Chemicals 1978, K2). The second study was conducted according to EU Method C2. The 48-h EC50 for mobility was determined to be > 500 mg/L (BASF AG 1988, K2).  In the study performed with substance CAS 147-14-8 according to OECD TG 202, the 48-h EC50 for mobility was determined to be > 500 mg/L (BASF AG 1989, K2). 

The determined LC50 values were significantly higher than the water solubility of the test substance. Based on the available information, it is concluded that all substances in this category are not acutely toxic to aquatic invertebrates, with an LC50 of 154 mg/L.