1,4-bis(p-tolylamino)anthraquinone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Data is from experimental lab report

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

To evaluate the test substance for its ability to generate acute toxic effects in Cyprinus carpio during an exposure period of 96 hours

GLP compliance:

not specified

Analytical monitoring:

not specified

Details on sampling:

No data available

Vehicle:

not specified

Details on test solutions:

test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes.Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/i solution appeared to be a black turbid dispersion with a floating layer. The test solution was used as such.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Source: Zodiac, proefacc, "De Haar Vissen", L.U. Wageningen, the Netherlands.
- Length at study initiation : 2.25 ± 0.19 cm
- Weight at study initiation :0.23 ± 0.06 g
- Method of breeding: F1 from a single parent-pair bred in UV-treated water

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

ISO-medium, hardness: 218 mg CaC03/l

Test temperature:

Between 19.0 and 21.6°C

Dissolved oxygen:

detailed in table no. 1

Nominal and measured concentrations:

100 mg/I.

Details on test conditions:

-Test vessels: 6 litres, all-glass
-Loading: 0.23 g fish per litre, i.e. 5 fish per 5 litres of test medium.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No mortality observed

Sublethal observations / clinical signs:

MACROLEX GREEN5B (mg/I)	Initial number of fish	Cumulative mortality						Total Mortality (%)
		3^1/4h	24 h	48 h	72 h	96 h
Blank- control	5	0	0	0^1)	0^1)	0^1)	0
100	5	0^#)	0^*)	0^*)	0^*)	0^*)	0

‘) :precipitate and floating layer observed

 

1): precipitate (probably fish faeces) observed

 

*)  floating layer observed.

Validity criteria fulfilled:

not specified

Conclusions:

Under the conditions of the present test material induced no mortality or other visible effects in carp at a nominal concentration of 100 mg/I during the 96-hour test period. Hence, the 96h-LC50 was above 100 mg/I, the regulatory limit concentration.

Executive summary:

Test material was evaluated for its toxicity on Cyprinus carpio as per OECD 203 and European Economic Community (EEC). Test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes. Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/i solution appeared to be a black turbid dispersion with a floating layer. The test solution was used as such.

Under the conditions of the present test material  induced no mortality or other visible effects in carp at a nominal concentration of 100 mg/I during the 96-hour test period. Hence, the 96h-LC50 was above 100 mg/I, the regulatory limit concentration.Based on the LC50 value it can be concluded that test material is not hazardous to fish and can not be considered to be not classified as per CLP criteria.

Description of key information

Short-term toxicity to fish:

Test material was evaluated for its toxicity on Cyprinus carpio as per OECD 203 and European Economic Community (EEC). Test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes. Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/i solution appeared to be a black turbid dispersion with a floating layer. The test solution was used as such.

Under the conditions of the present test material  induced no mortality or other visible effects in carp at a nominal concentration of 100 mg/I during the 96-hour test period. Hence, the 96h-LC50 was above 100 mg/I, the regulatory limit concentration.Based on the LC50 value it can be concluded that test material is not hazardous to fish and can not be considered to be not classified as per CLP criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Short-term toxicity to fish:

In an experimental study test material was evaluated for its toxicity on Cyprinus carpio as per OECD 203 and European Economic Community (EEC). Test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes. Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/i solution appeared to be a black turbid dispersion with a floating layer. The test solution was used as such.

Under the conditions of the present test material  induced no mortality or other visible effects in carp at a nominal concentration of 100 mg/I during the 96-hour test period. Hence, the 96h-LC50 was above 100 mg/I, the regulatory limit concentration.

In another experimental report , study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The stock solution was prepared by dissolving 0.1gm of the test substance in 100mL of potable water (passed through reverse osmosis system) and kept it for 24 hrs stirring. By giving half an hour of settling period filtered it . The nominal concentration were 0.45 mg/L, 0.9 mg/L, 1.8mg/L, 3.6mg/L, 7.2 mg/L. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC0 was determine to be 7.2 mg/l . No mortalities were observed in any of the tested concentration.

Based on the LC50 value it can be concluded that test material is not hazardous to fish and can not be considered to be not classified as per CLP criteria.