Succinimide

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Study period:

not reported

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

unsuitable test system

Qualifier:

no guideline followed

Principles of method if other than guideline:

Toxicity towards microorganism was tested with the in vitro testing system Microtox model 500. The tests were performed following the Acute Detailed Basic Testing procedure presented by AZUR Environmental.
The principle of the microtox assay is based on measuring the bioluminescence produced by the bacteria Allivibrio fischeri, formerly known as Vibrio fischeri. Light is emitted as a by-product of metabolic processes from A. fischerii. Therefore, an inhibition of metabolism by toxic substances leads as well to an inhibition of luminescence.

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

"Chemicals used in this study include NMP and its oxidative byproducts: methylsuccinimide, 2-pyrrolidinone, and succinimide. All compounds were of the highest purity grade. Test samples were prepared by diluting the pure compounds with deionized water."

Test organisms (species):

Vibrio fisheri

Details on inoculum:

Microtox utilizes the lyophilized marine microorganism Vibrio fisheri (Northern Regional Research Laboratory strain B-1 1 177).
Actual process water was obtained from the Marine Corps Multi-Commodity Maintenance Centers.

Test type:

static

Water media type:

saltwater

Total exposure duration:

30 min

Remarks on exposure duration:

The 30-min test was used due to the fact that the 5- and 15-min EC50 values for the process water showed a larger error (i.e., larger 95% confidence intervals) than those obtained for the 30-min test times.

Nominal and measured concentrations:

100.0, 50.0, 25.0, 20.0, 15.0, 12.5, 5.00, 4.75 (nominal)

Reference substance (positive control):

not specified

Duration:

5 min

Dose descriptor:

EC50

Effect conc.:

14.2 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: inhibition of luminescence

Key result

Duration:

5 min

Dose descriptor:

EC50

Effect conc.:

13.72 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: inhibition of luminescence

Duration:

5 min

Dose descriptor:

EC50

Effect conc.:

14.1 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: inhibition of luminescence

Duration:

5 min

Dose descriptor:

EC50

Effect conc.:

15.83 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: inhibition of luminescence

Duration:

5 min

Dose descriptor:

EC50

Effect conc.:

15.9 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: inhibition of luminescence

Validity criteria fulfilled:

not specified

Conclusions:

The EC50 value of succinimide towards Allivibrio fischeri is approximately 14.75 g/L.

Executive summary:

In a Microtox toxicity assay the 5 -min EC50 value of succinimide towards the bioluminescent bacterium Allivibrio fischeri was determined with a mean value of 14.75 g/L.

Description of key information

The 5 min. EC₅₀ value of succinimide towards Allivibrio fischeri is approximately 14.75 g/L.

Key value for chemical safety assessment

EC50 for microorganisms:

14.75 g/L

Additional information

Allivibrio fischeri requires a high salinity environment, therefore this information is not of high relevance for common STP, but is presented as additional information.