Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-589-7 | CAS number: 122-99-6
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Testing of the toxicity of 2-phenoxyethanol to microorganisms yielded an EC10(30 min) of 360 mg/L regarding activated sludge respiration inhibition.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 360 mg/L
Additional information
The inhibiting effect of 2-phenoxyethanol (purity > 99.9 area-%) on oxygen consumption of activated sludge was investigated in an activated sludge respiration inhibition test according to OECD guideline 209 (1993), Annex to EEC Directive 88/302/EEC (1987), ISO Standard 8192-1986 (E, Method B) and EPA OPPTS 850.6800 (1996) at following concentrations: 0, 248, 496 and 1000 mg/L (BASF AG, 2002). After an incubation time of 30 min, the concentration of dissolved oxygen was measured at test start and after 6 min. Effect concentrations (EC20, EC50, EC80) were obtained from graphical interpolation, resulting in EC20, EC50and EC80 values of 620 mg/L, > 1000 mg/L and > 1000 mg/L, respectively. The data was recalculated resulting in a statistical EC10 of 360 mg/L (not reported in the study).
Another key study investigated the toxicity of 2-phenoxyethanol to the bacterium Pseudomonas putida in a bacterial growth inhibition test according to DIN guideline 38412 Part 8 (Sasol Germany GmbH, 1994), with nominal concentrations of 0, 187.5, 375, 750, 1500, 3000 and 6000 mg/L. After 16 h, an EC10 of 410 mg/L and an EC50 of 1494 mg/L was determined.
In a third key study, the toxicity of 2-phenoxyethanol to the bacterium Pseudomonas putida was determined in a bacterial growth inhibition test according to DIN guideline 38412 Part 8, draft (BASF AG, 1989). Growth inhibition was measured at nominal concentrations of 0, 78.13, 156.25, 312.5, 625, 1250, 2500 and 5000 mg/L after 17 hours of incubation, resulting in an EC10 of 318.9 mg/L, an EC50 of 883.3 mg/L and an EC90 of 2129.5 mg/L.
Further, a supporting study examined growth inhibition effects on Pseudomonas putida and aerobic microorganisms with resulting EC50 value of 1650 mg/L. Another study evaluated reduced light output of Photobacterium phosphoreum with an resulting EC50 after 5 minutes of 32.7 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
