Vanillic acid

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29-11-2002 to 28-12-2002

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Screening test.

Qualifier:

according to guideline

Guideline:

ISO 14593:1999 (Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test))

Version / remarks:

Screening test.

GLP compliance:

not specified

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 01JAT1272
- Expiration date of the lot/batch: no data
- Purity test date: no data

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at 4°C.
- Stability under test conditions: yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, adapted

Details on inoculum:

Preparation of activated sludge: The inoculum was prepared from activated sludge taken from a domestic effluent treatment plant (Jundiai, Brazil). Coarse particles have been removed by filtration. The sludge was then centrifuged and washed and the pellets returned suspended in culture medium. They were used as inoculum at a concentration of 4 mg / l (dry matter).
No other information on the inoculum available.

Duration of test (contact time):

28 d

Initial conc.:

20 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

TOC removal

Details on study design:

TEST CONDITIONS: no data
TEST SYSTEM
- Volume of test solution: 120 mL
- Number of culture flasks/concentration: 2
- magnetic stirrer
- Test temperature: 22°C

SAMPLING: No data

CONTROL AND BLANK SYSTEM
- Inoculum blank: 1 test vessel with 120 mL of basal culture medium (sludge)
- sludge + test substance at 20 mg/L
- reference substance: sludge + sodium benzoate at 20 mg/L
- Concentration of activated sludge (as suspended solid concentration): 20 mg/l

Reference substance:

other: Sodium Benzoate

Key result

Parameter:

% degradation (TOC removal)

Value:

97.5

Sampling time:

28 d

Key result

Parameter:

% degradation (TOC removal)

Value:

73

Sampling time:

5 d

Results with reference substance:

% degradation (TOC removal): 96.9% after 28 days.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The corresponding percentages of biodegradation obtained after 5 and 28 days were 73% and 97.5% respectively. Under the test conditions of this study, Vanillic acid is therefore considered as readily biodegradable.

Executive summary:

The biodegradation of Vanillic acid (initial concentration of 20 mg/L) was studied during 28 days according to ISO 14593 (Reliability 2, Not GLP, Solvay Internal Report). Inoculum used in the test was an activated sludge at a concentration of 4 mg/Lpreparedfrom activated sludge taken from an aeration tank of a domestic effluent treatment plant (Jundiai - Brazil).

The percentage of biodegradation was estimated by the Total Organic Carbon (TOC). The corresponding percentages of biodegradation obtained after 5 and 28 days were 73% and 97.5% respectively. Under the test conditions of this study, Vanillic acid is therefore considered as readily biodegradable.

Description of key information

One study was available and considered as the key study. The biodegradation of Vanillic acid (initial concentration of 20 mg/L) was studied during 28 days according to ISO 14593 (Reliability 2, Not GLP). Inoculum used in the test was an activated sludge at a concentration of 4 mg/Lprepared from activated sludge taken from an aeration tank of a domestic effluent treatment plant (Jundiai, Brazil).

The percentage of biodegradation was estimated by the Total Organic Carbon (TOC). The corresponding percentages of biodegradation obtained after 5 and 28 days were 73% and 97.5% respectively. Under the test conditions of this study, Vanillic acid is therefore considered as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable