3-amino-4-methoxybenzanilide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: According to national standards (DIN 10707:1998-03)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1989

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: DIN EN ISO 10707

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-amino-4-methoxybenzanilide

EC Number:

204-388-4

EC Name:

3-amino-4-methoxybenzanilide

Cas Number:

120-35-4

Molecular formula:

C14H14N2O2

IUPAC Name:

3-amino-4-methoxy-N-phenylbenzamide

Study design

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- origin: sewage treatment plant at Frankfurt am Main, Sindlingen (domestic)
- type of inoculum: supernatant from settled domestic sewage sludge, no pre-treatment, not pre-adapted to the test or the reference substance

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

>= 2.58 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

- Test temperature: 20°C ± 1°C
- Culturing apparatus: 250 mL BOD bottles
- Sampling method: for each oxygen measurement a separate vessel was used. The vessels were not further incubated after they had been opened for one analysis
- Degradation analysis: at each sampling time the dissolved oxygen content was measured in 3 vessels.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

7 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

14 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

2

Sampling time:

21 d

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The degradation of the reference substance sodium benozoate reached > 70 % in 7 days. Details see below.

Any other information on results incl. tables

Table 1: Results test substance

Time	O2-concentration [mg/L], mean values
	Test substance	Control	O2-consumption	O2-consumption in [%] of the COD
0 d	8.57	8.58	0.01	0
7 d	8.53	8.29	-0.06	0
14 d	8.04	8.04	0.00	0
21 d	7.99	8.09	0.10	2
28 d	8.15	7.76	-0.39	0

Table 2: Results reference substance

Time	O2-concentration [mg/L], mean values
	Reference substance	Control	O2-consumption	O2-consumption in [%] of the COD
0 d	8.83	8.58	-0.25	0
7 d	4.47	8.29	3.83	74
14 d	4.40	8.04	3.64	70
21 d	4.38	8.09	3.71	72
28 d	4.61	7.76	3.51	61

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test substance is not readily biodegradable.

Executive summary:

During a test period of 28 days no significant oxygen consumption could be noticed. Test revealed, that according to OECD 301D Guideline the test substance is not readily biodegradable.