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Diss Factsheets

Administrative data

Link to relevant study record(s)

Description of key information

EC50 (14 d): 120 mg a.i./kg dw soil (fresh weight, Avena sativa, OECD 208, Bützler 2007)

Key value for chemical safety assessment

Short-term EC50 or LC50 for terrestrial plants:
120 mg/kg soil dw

Additional information

A test according to OECD guideline 208 was conducted over 14 days (after 50% emergence of the seedlings in the control group) in treated natural soil testing effects of the test item Chlorophene (CAS 120-32-1) to different plant species (Bützler, 2007). Three plant species out of three different families were tested. The dicotyledon Brassica napus was tested with the nominal test item concentrations of 68.3, 150, 331 and 727 mg/kg dw, the dicotyledon Glycine max with the nominal test item concentrations of 68.3, 150, 331, 727 and 1600 mg/kg dw, and the monocotyledon Avena sativa with the nominal test item concentrations of 6.41, 14.1, 31.0, 68.3, 150, 331 and 727 mg/kg dw (each test range with the spacing factor 2.2). Six replicates per treatment each with five seeds were used. The test item was dissolved in acetone and mixed with quartz sand; the treated sand was mixed into the soil. The analysis of the test item (HPLC) resulted in recovery rates of 80% and 85% of the nominal values, thus, for calculations of the effect concentrations the nominal concentrations were used. Measured endpoints in this study were ‘fresh weight, germination, mortality and phytotoxicity. The EC50 based on the endpoint ‘fresh weight’. The results showed for the endpoint ‘fresh weight’ an effect (LOEC) at the test concentration 150 mg/kg dw soil with the test plant Avena sativa, the most sensitive species in this study. The EC50 was calculated with 120 mg a.i./kg dw soil. Brassica napus followed with an effect concentration of EC50 235 mg a.i./kg dw soil. Less sensitive was Glycine max which showed an EC50 of 546 mg a.i./kg dw soil. For the risk assessment, the lowest EC50 value of 120 mg a.i./kg dw soil is used.