trans-crotonic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996-01-19 to 1996-01-21

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 4049692

Details on sampling:

- sampling time: 0 hour (test initiation) and at 48 hours (test termination)
- Sampling method:Each exposure solution sample was collected from the approximate midpoint of the test vessel by volumetric pipet.

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
Stock solution:
400 mg/mL stock solution: 20.02 g of test substance with methanol (CAS 67-56-1) to volume in a 50-mL volumetric flask.
0.32 mL/mL solvent stock solution: diluting 16 mL of methanol with distilled water to a volume of 50 mL in a volumetric flask.
Test system:
A 50-mL Hamilton® gas tight syringe

- vehicle: methanol
- Controls: diluted water

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source:cultures maintained at Springborn.
- Age at study initiation: < 24 hours old
- Food type: Unicellular green algae, Ankistrodesmus falcatus
- Amount: 2.0 mL
- Frequency: Once daily, Daphnids were not fed during the 48-hour exposure

Test type:

other: continuous-flow

Total exposure duration:

48 h

Hardness:

160-170 mg/L CaC03

Test temperature:

20-21°C

pH:

5.4-8.2

Dissolved oxygen:

7.7-8.7 mg/L

Salinity:

Total Alkalinity: 86-110 mg/L CaCO3

Nominal and measured concentrations:

Nominal concentration: 13, 25, 50, 100 and 200 mg/L
Exposed concentration: 18, 35, 59, 110 and 200 mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: 14
- Type: closed
- Aeration: continuously aerated
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (diluted water): 2
- No. of vessels per vehicle control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Total organic carbon: 0.96 mg/L
- Metals: no toxic concentration
- Pesticides: no toxic concentration
- Alkalinity: 110 mg/L (CaCO3)


OTHER TEST CONDITIONS

- Photoperiod: 16 hours light and 8 hours darkness
- Light intensity: 45 to 80 footcandles

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

150 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

110 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Following 24 hours of exposure, 100% immobilization was observed among daphnids exposed to the highest concentration tested, 200 mg/L. Following 48 hours of exposure, no immobilization was observed among daphnids exposed to the remaining treatment levels tested, 18, 35, 59 and 110 mg/L. During the same period, 5 and 0% immobilization was observed among daphnids exposed to the control and solvent control, respectively. No sublethal effects (e.g., lethargy) were observed among daphnids exposed to the concentration range tested or the controls.

Conclusions:

Under the conditions of the present acute toxicity study to aquatic invertebrates (Daphnia magna) with Crotonic acid after an exposure time of 48h a EC50 of 150 mg/L and a NOEC of 110 mg/L was determined.

Description of key information

Acute toxicity to invertebrates: EC50 value was 150 mg/L and NOEC was 110 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

150 mg/L

Additional information

The acute toxicity of Crotonic acid to Daphnia magna (water flea) was tested under flow-through conditions for 48 hours in accordance with OECD guideline no. 202 and EU method C.2 (Springborn Laboratories (b), 1997). Crotonic acid was diluted with methanol and tested in the nominal concentrations of 13, 25, 50, 100 and 250 mg/L (exposure concentrations: 18, 35, 59, 110 and 200 mg/L). Following 24 hours of exposure, 100% immobilization was observed among daphnids exposed to the highest concentration tested, 200 mg/L. Following 48 hours of exposure, no immobilization was observed among daphnids exposed to the remaining treatment levels tested, 18, 35, 59 and 110 mg/L. During the same period, 5 and 0% immobilization was observed among daphnids exposed to the control and solvent control, respectively. No sublethal effects (e.g., lethargy) were observed among daphnids exposed to the concentration range tested or the controls. In conclusion, under the conditions of the present acute toxicity study to aquatic invertebrates (Daphnia magna) with Crotonic acid after an exposure time of 48h an EC50 of 150 mg/L and a NOEC of 110 mg/L was determined.