Isopentyl propionate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reveiwed journal.

Justification for type of information:

Data is from peer reveiwed journal.

Qualifier:

according to guideline

Guideline:

other: As mention below

Principles of method if other than guideline:

In order to discover the antimicrobial activity against skin resident microorganisms responsible for axillary odor, the minimum inhibitory concentrations (MICs) of common fragrance materials were determined by agar plate dilution method.

GLP compliance:

not specified

Specific details on test material used for the study:

Name of test material (as cited in study report): Isoamyl propionate
Molecular formula (if other than submission substance): C8H16O2
Molecular weight (if other than submission substance): 144.212
Smiles notation (if other than submission substance): C(OCCC(C)C)(CC)=O
InChl (if other than submission substance):
1S/C8H16O2/c148(9)10657(2)3/h7H,46H2,13H3
Substance type: Organic
Physical state: Liquid

Analytical monitoring:

yes

Details on sampling:

Sampling method: Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.
Sample storage conditions before analysis: No data

Vehicle:

yes

Details on test solutions:

Vehicle- Ethyl alcohol or dimethyl sulfoxide (DMSO).

Test organisms (species):

other: Corynebacterium minutissimum (CM) Arthrobacter sp, Staphylococcus aureus (IAM-1011, (SA)), Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC))

Details on inoculum:

Corynebacterium minutissimum (ATCC 23348, (CM)), was gifted from the Department of Dermatology, University of Pennsylvania.
Arthrobacter sp. isolated from Lipo-66 is a geophilic organism.
Staphylococcus aureus (IAM-1011, (SA)) was purchased from Institute of Applied Microbiology, Tokyo university
Staphylococcus epidermidis var. (SE) was gifted from the Department of Dermatology, University of Pennsylvania
Escherichia coli (ATCC 11775, (EC)) was purchased from Institute of Medical Science, Tokyo university.

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

24 h

Remarks on exposure duration:

No data available

Post exposure observation period:

No data available

Hardness:

No data available

Test temperature:

37◦ C

pH:

7.6-8

Dissolved oxygen:

No data available

Salinity:

No data available

Conductivity:

No data available

Nominal and measured concentrations:

No data available

Details on test conditions:

Test vessel: Agar plates
Material, size, headspace, fill volume:
Aeration: No data
Type of flow-through (e.g. peristaltic or proportional diluter): No data
Renewal rate of test solution (frequency/flow rate): No data
No. of organisms per vessel: microbial concentration of 106CFU/ml
No. of vessels per concentration (replicates):
No. of vessels per control (replicates): No data
No. of vessels per vehicle control (replicates): No data
Biomass loading rate: No data

Reference substance (positive control):

not specified

Key result

Duration:

24 h

Dose descriptor:

other: MIC

Effect conc.:

> 2 000 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: For all species

Remarks:

Non toxic

Details on results:

No data available

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

No data available

Validity criteria fulfilled:

not specified

Conclusions:

The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM), Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) and Arthrobacter species was >2000 mg/l (2000ppm) (inoculum 105CFU/plate) after 24 hours exposure to isoamyl propionate.

Executive summary:

Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 10⁶CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.

 

The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM), Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) and Arthrobacter species was>2000 mg/l (2000ppm) (inoculum 10⁵CFU/plate) after 24 hours exposure to isoamyl propionate.

Description of key information

Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 10⁶CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.

The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM), Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) and Arthrobacter species was >2000 mg/l (2000ppm) (inoculum 10⁵CFU/plate) after 24 hours exposure to isoamyl propionate.

Additional information

Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 10⁶CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.

The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM), Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) and Arthrobacter species was >2000 mg/l (2000ppm) (inoculum 10⁵CFU/plate) after 24 hours exposure to isoamyl propionate.