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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
p-mentha-1,4-diene
EC Number:
202-794-6
EC Name:
p-mentha-1,4-diene
Cas Number:
99-85-4
Molecular formula:
C10H16
IUPAC Name:
p-mentha-1,4-diene
Specific details on test material used for the study:
Name: P-MENTHA-1,4 DIENE (GAMMA TERPINENE)
Batch: 161215
Preparation date: 15/12/2016
Expiration date: 14/12/2018
Receiving date: 06/04/2017
Chelab ID: 17.588206.0002
Composition as reported in the certificate provided by the Sponsor (see annex n° 1 to the Study Plan):
- P-MENTHA-1,4 DIENE (GAMMA TERPINENE) (fragrance: pure) CAS 99-85-4
Storage conditions: cool, dry place, protected from fire.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
As test system the fish species Brachydanio rerio was employed in the assay, as described by the guideline OECD
203:1992. The animals had a maximal length of 2.0 cm±1.0 cm at the beginning of the test. they were in good
health and free from any apparent malformation, as indicated in the veterinary Certificate.
For this test, the following fish batch was used: 02/2017.

Study design

Water media type:
other: dilution water
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
The water used in the test had hardness 28.51mg, expressed as Ca content per liter(acceptance criteria 10< Ca<250mg)
Test temperature:
20-25Centigrade
pH:
6-8.5
Dissolved oxygen:
>60%
Conductivity:
the deionized water had a conductivity <=10μS/cm
Nominal and measured concentrations:
Nominal concentration: 750mg/L

Results and discussion

Effect concentrations
Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
2.792 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes