Registration Dossier
Registration Dossier
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EC number: 202-551-4 | CAS number: 97-00-7
Long-term toxicity to fish
Administrative data
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: conforms to guideline & GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1-chloro-2,4-dinitrobenzene
- EC Number:
- 202-551-4
- EC Name:
- 1-chloro-2,4-dinitrobenzene
- Cas Number:
- 97-00-7
- Molecular formula:
- C6H3ClN2O4
- IUPAC Name:
- 1-chloro-2,4-dinitrobenzene
- Details on test material:
- Analytical purity: 99.7 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Substance concentration determined before study and once a week until the end
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Total exposure duration:
- 28 d
Test conditions
- Hardness:
- 2.5 - 2.7 mmol/l
- Test temperature:
- 21 - 23 ºC
- pH:
- 7.9 - 8.3
- Details on test conditions:
- Conductivity: 630 - 700 uS/cm
Photoperiod: 6 am to 6 pm
Light intensity: approx. 700 lux
Test concentrations: 0 / 0.005 / 0.016 / 0.05 / 0.16 / 0.5 mg/l (10 fish used per concentration)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.16 mg/L
- Basis for effect:
- mortality
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.05 mg/L
- Basis for effect:
- mortality
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.05 mg/L
- Basis for effect:
- other: any observed effect
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.016 mg/L
- Basis for effect:
- other: any observed effect
- Details on results:
- No lethality occurred in the concentration of 0.05 mg/l or less.
- Results with reference substance (positive control):
- No lethality occurred in the control group.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
