4-amino-2-methylpyrimidine-5-methylamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20/02/2001 - 23/02/2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed under GLP according to OECD guideline 202

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-amino-2-methylpyrimidine-5-methylamine

EC Number:

202-384-7

EC Name:

4-amino-2-methylpyrimidine-5-methylamine

Cas Number:

95-02-3

Molecular formula:

C6H10N4

IUPAC Name:

5-(aminomethyl)-2-methylpyrimidin-4-amine

Details on test material:

- Name of test material (as cited in study report): Grewe-diamine
- Stability under test conditions: stable
- Storage condition of test material: room temperature

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 128 - 132°C
- Water solubility (under test conditions): 430 mg/l



OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: not readily biodegradable

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

not relevant

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

24 h

Results and discussion

Effect concentrations

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

No behavioural abnormalities or immobilization was determined. The results on the reference substance kaliumdichromate showed an immobilization of Daphnia (EC50 1.82 mg/l).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 24h EC50 was determined to >1000 mg/l, the 24 h EC50 (reference substance) = 1.82 mg/l.

Executive summary:

A range-finding test was followed by a EC50 limit test performed under static conditions for 24 hours of exposure. In the EC50 limit test Daphnia were exposed for 24 hours to 1000 mg/l.

The study included a blank-control. The test was performed in quadruplicate with 5 daphnia

per vessel. The 24h EC50 was determined to >1000 mg/l, the 24 h EC50 (reference substance) = 1.82 mg/l.