Naphthalene-1,8-dicarboximide

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Naphthalene-1,8-dicarboximide

EC Number:

201-379-7

EC Name:

Naphthalene-1,8-dicarboximide

Cas Number:

81-83-4

Molecular formula:

C12H7NO2

IUPAC Name:

3-azatricyclo[7.3.1.0⁵,¹³]trideca-1(13),5,7,9,11-pentaene-2,4-dione

Details on test material:

- Name of test material (as cited in study report): NAPHTHALIMID TROCKEN BER. 100%
- Lot/batch No.:P.265
- Analytical purity: 99.6 %

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

activated sludge

Details on inoculum:

The activated sludge was taken from a Iaboratory wastewater plants treating municipal sewage.
- Initial biomass concentration: 1 g/l

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

180 min

Test conditions

Test temperature:

20 ± 2 °C

pH:

6.7 before correction
7.1 after correction

Nominal and measured concentrations:

Nominal test substance concentration: 1002 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium: 8 ml/vessel 1 00-fold concentrated OECD medium

Reference substance (positive control):

yes

Remarks:

3,5-dichlorphenole

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

180 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

180 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

180 min

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

Change in oxygen consumption rate compared with blank control after 180 min 1002 mg/l --> -6 % (respiration stimulation)