Phenethylamine

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984-11-20 untill 1984-11-24

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure according to national standards

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German industrial standard test guideline DIN 38 412, part L15.

Version / remarks:

June 1982

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Phenethylamine

EC Number:

200-574-4

EC Name:

Phenethylamine

Cas Number:

64-04-0

Molecular formula:

C8H11N

IUPAC Name:

2-phenylethanamine

Specific details on test material used for the study:

Test substance no.: 84/416

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test solution was prepared as an aqueous solution. The product was added to the test water and the fish were placed subsequently into the aquaria.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Goldorfe
- Strain: Leuciscus idus (golden variety)
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation: 7.6 cm (range: 6.5-8.6)
- Weight at study initiation: 3.6 g (range: 1.9-5.3)
- Feeding during test: no
- Frequency: Withdrawal of food before exposure: 1 day

ACCLIMATION
- Acclimation conditions (same as test or not): oil-free aerated and charcoal filtered tap water, flow-through system
Water temperature: 19-21°C
Acclimation to test water and test temperature: 3 days

- Type and amount of food: "Fukosalm" - growing feed 1; Fuko - Kraftfutterfabrik, 7900 Ulm, Germany
- Feeding frequency: ad libitum.

HOUSING
- Housing water: charcoal filtered dechlorinated water in continuous flow-through
- Aeration: oilfree air
- Light regime: 16 : 8 hours light : dark
- Hardness: 2.6 mM
- Oxygen concentration: > 60 % of maximum saturation
- pH: about 7.5
- Water temperature: 19 - 21 °C
- Duration of housing: about 4 month
- Medical treatment: four times with 0.05 mg/L Malachitegreen and three times with 10 mg/L tetracyclinhydrochlorid
- Feeding: "Fukosalm" - growing feed 1; Fuko - Kraftfutterfabrik, 7900 Ulm, Germany
- Amount of feed: ad libitum
- Mortality during the last week of housing: 0.2 %

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness: 2.5 mmol/L

Test temperature:

20 ± 1 °C

pH:

about 7.8

Nominal and measured concentrations:

Nominal concentrations: 10, 14.7, 21.5, 31.6, 46.4, 68.1 mg/L. Additionally: Neutralised samples (68.1 mg/L)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: aquaria open
- Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L
- Aeration: aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 3.6 g fish /L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Ca/Mg ratio: 4 : 1
- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light:dark

EFFECT PARAMETERS MEASURED: mortality, symptoms

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

68.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: neutralised test solution

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

38.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: not neutralised test solution

Remarks:

geometric mean of LC0 (31.6 mg/L) and LC100 (46.4 mg/L)

Details on results:

- Behavioural abnormalities: no symptoms detectable
- The pH of the test solutions >= 31.6 mg/L were above the preferred pH range (pH 6.0 to 8.5) described in OECD 203. Therefore, pH efects are likely.
- The highest test solution (68.1 mg/L) was also tested after pH-adjustment with HCl (20%). The substance was tolerated without any mortality or any symptoms being observed.

Reported statistics and error estimates:

The LC50 was determined by forming the geometric mean between the the highest concentration causing no mortality and the lowest concentration producing 100% mortality. This method is described in OECD 203 were data are inadequate to use other statistical methods.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Results

Nominal concentration [mg/L]	no. of fish (beginning)	dead fish after
		1 h	4 h	24 h	48 h	72 h	96 h
10.0	10	0	0	0	0	0	0
14.7	10	0	0	0	0	0	0
21.5	10	0	0	0	0	0	0
31.6	10	0	0	0	0	0	0
46.4	10	10	10	10	10	10	10
68.1	10	10	10	10	10	10	10
68.1*	10	0	0	0	0	0	0

*: Test solution was pH-adjusted.

pH values

Test item conc.	pH
(mg/L)	initial	24 h	48 h	72 h	96 h
0	7.6	7.6	7.5	7.7	7.6
10	7.5	7.3	7.3	7.5	7.5
14.7	8.0	7.1	7.0	7.2	7.2
21.5	8.4	7.1	7.1	7.3	7.2
31.6	8.7	7.0	7.1	7.4	7.3
46.4	9.2
68.1	9.5
68.1*	7.4	7.3	7.3	7.5	7.4

Dissolved oxygen

Test item conc.	Oxygen content (mg/L)
(mg/L)	1 h	24 h	48 h	72 h	96 h
0	8.3	8.4	8.7	8.7	8.7
10	7.6	8.3	8.4	8.4	8.6
14.7	7.4	7.0	6.9	7.3	7.5
21.5	7.3	6.5	7.0	7.5	7.3
31.6	6.9	5.8	6.2	7.3	7.3
46.4	8.8
68.1	8.9
68.1*	8.3	8.7	8.8	8.7	8.7