[No public or meaningful name is available]

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: secondary literature

Data source

Reference

Reference Type:

secondary source

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Principles of method if other than guideline:

The test material was dispensed in corn oil and administered to one rat per dose per intragastric intubation. Dose rates administered ranged from 200 to 3400 mg/kg bw in increments of approx. 50%. Following administration of the test substance, rats were observed for clinical signs and toxicity. Observations for mortality were made daily throughout the study.

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

not available

EC Number:

691-232-7

Cas Number:

474266-68-7

Molecular formula:

not available

IUPAC Name:

not available

Details on test material:

composition:
approx. 20% diisobutylene nitrosate
approx. 80% diisobutylene

Test animals

Species:

rat

Strain:

other: Crl:CD BR

Sex:

male

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Doses:

200, 300, 450, 670, 1000, 1500, 2300, 3400 mg/kg bw

No. of animals per sex per dose:

one male rat/dose

Control animals:

not specified

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

other: ALD = approximate lethal dose

Effect level:

450 mg/kg bw

Based on:

test mat.

Any other information on results incl. tables

The lowest dose of diisobutylene nitrosate where one animal died was 450 mg/kg bw. The survival of the rats dosed at 670 mg/kg bw or 1000 mg/kg bw was attributed to animal variability and does not change the ALD (approximate lethal dose).

The rats dosed at 200 and 300 mg/kg bw exhibited no clinical signs of toxicity during the study. The rats dosed at 670 mg/kg bw exhibited a lump (approx. 1 cm diameter) in the abdominal area from test day 8 through the end ofthe study. The rat dosed at 1000 mg/kg bw exhibited lethargic behaviour, convulsions, red nasal discharge, ruffled fur, diarrhea and yellow stained perineum. Slight to severe weight loss (up to 12% of initial body weight) were observed in most rats one day after dosing. The rats dosed at 450, 1500 or 3400 mg/kg bw exhibited convulsions approximaetly 15 minutes after dosing. The rat dosed at 2300 mg/kg bw did not exhibit any clinical signs before death. All deaths occured within 2 hours after dosing.

Applicant's summary and conclusion

Executive summary:

Diisobutylene nitrosate (approximately 20% pure) was administered as a single oral dose by gastric intubation to male rats. Deaths occured within 2 hours after dosing. Clinical signs of toxicity were observed in lethally and nonlethally dosed animals. Under the condition of this test, the ALD was 450 (approximately lethal dose) mg/kg bw.