Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 931-105-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- november 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of L-isoleucine and octadecan-1-ol and ethanesulphonic acid and docosan-1-ol
- EC Number:
- 931-105-8
- Cas Number:
- 1156505-34-8
- Molecular formula:
- C26-30H56-64O5NS
- IUPAC Name:
- Reaction mass of L-isoleucine and octadecan-1-ol and ethanesulphonic acid and docosan-1-ol
Constituent 1
- Specific details on test material used for the study:
- Test item Brassicyl Isoleucinate Esylate
Batch # D-570-069
Expiration date August 2011
Storage conditions Room temperature
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The Daphnia magna used in this toxicity test were obtained from laboratory cultures maintained at Springborn Smithers Laboratories (Europe), originally obtained from a culture at Springborn Smithers Laboratories Wareham (MA), USA.
Prior to testing, the daphnids were held in glass beakers under a photoperiod of 16 hours light and 8 hours darkness with a 30 minute transition period. The culture water consisted of modified Elendt M4 medium. After preparation the medium was aerated using an air pump to bring the pH and dissolved gases into equilibrium with the atmosphere. The modified Elendt M4 medium was allowed to equilibrate for at least two days prior to use. Total hardness, total alkalinity, pH and specific conductivity of the dilution water were determined after preparing each batch to verify that these parameters are within the acceptable ranges. Representative samples of the dilution water source used in the preparation of the culture medium were analyzed half-yearly for the presence of pesticides, PCBs and toxic metals at Bachema, Schlieren, and Interlabor Belp AG, Belp, Switzerland. None of these compounds have been detected at concentrations that are considered toxic in any of the water samples analyzed. Based on the results of these analyses and the ability of daphnids to survive and reproduce over multiple culture generations in the dilution water, it was confirmed that the dilution water used was of acceptable quality.
The batch of dilution water used in the culture three days prior to test initiation was characterized as having a total hardness and a total alkalinity as calcium carbonate (CaCO3) of 170 mg/L and of 28 mg/L, respectively, a specific conductivity of 420 μS/cm and a pH of 6.61. The temperature ranged from 19.8 to 21.0°C three days prior to test initiation.
Daphnids were fed 2.0-2.5 mL of a solution containing approximately 4 x 107 cells/mL of the unicellular green alga, Ankistrodesmus falcatus (ANK) and approximately four drops or 0.5 mL of a combination of yeast, cereal leaves and flaked fish food (YCT) daily. Representative samples of the food were analyzed periodically for the presence of pesticides, PCBs and toxic metals at Bachema, Schlieren, and Interlabor Belp AG, Belp, Switzerland. None of these compounds have been detected at concentrations that are considered toxic.
Study design
- Test type:
- static
- Water media type:
- other: Modified Elendt M4 Medium pH: 6.61 Specific conductivity: 420 μS/cm Total hardness as CaCO3: 170 mg/L Total alkalinity as CaCO3: 28 mg/L
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Total hardness as CaCO3: 170 mg/L
- Test temperature:
- continuously measured temperature of 20.1 to 20.5oC (single point measured temperature 20.4 to 20.9ºC)
- pH:
- pH: 6.61
- Conductivity:
- Specific conductivity: 420 μS/cm
- Nominal and measured concentrations:
- definitive test concentrations of 6.25, 12.5, 25.0, 50.0 and 100 mg test item/L and a control were selected for the definitive exposure.
- Details on test conditions:
- The test vessels were placed in a water bath in order to maintain exposure solution temperatures at 20 ± 1°C. The test vessels were 250 mL glass beakers, each containing 200 mL of test solution. The test vessels were covered with a cling film during the 48 hour exposure. The test was illuminated to a light intensity of 399 to 475 lux using fluorescent bulbs. A 16-hour light, 8-hour dark photoperiod was maintained with an automatic timer.
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 > 100 mg/L
The highest concentration tested producing no immobile daphnids was determined to be 100 mg test item /L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.