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Reaction mass of Sodium [2,4-dihydro-4-[(2-hydroxy-5-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-,Sodium bis[1-[(2-hydroxy-5-nitrophenyl)azo]naphthalen-2-olato(2-)]cobaltate(1-)and Sodium bis[2,4-dihydro-4-[(2-hydroxy-5-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)]cobaltate(1-)
EC number: 916-867-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin irritation/corrosion: The test item has no skin irritation potential in an in vitro study according OECD TG 431.
Eye irritation: The test item has no eye irritation potential in an in vitro study according OECD TG 492.
Key value for chemical safety assessment
Skin irritation / corrosion
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Skin irritation/corrosion
The skin irritation and corrosion potential of the test substance was assessed in an in vitro test strategy. Two in vitro assays were part of this strategy: The Skin Corrosion Test (OECD TG 439, SCT) and Skin Irritation Test (OECD TG 431,SIT). However, in the current case the results derived with the Irritation test alone were sufficient for a final assessment. Therefore, further testing in SCT was waived.
In the study according OECD TG 431, the test item was applied using ca. 25 μL bulk volume (about 23 mg) of the undiluted test substance to a reconstructed three-dimensional human epidermis model (EpiDermTM). The irritation test was performed with three EpiDerm tissues which were incubated with the test substance for 1 hour followed by a 42-hour post-incubation period.
Tissue destruction was determined by measuring the metabolic activity of the tissue after exposure/post-incubation by using a colorimetric test. The reduction of mitochondrial dehydrogenase activity measured by reduced formazan production after incubation with a tetrazolium salt (MTT) was chosen as endpoint. The formazan production of the test substance treated epidermal tissues is compared to that of negative control tissues. The quotient of the values indicates the relative tissue viability.
Due to the color of the test substance, it could not be determined whether the test substance can directly reduce MTT. Therefore, an additional MTT reduction control (freeze-killed control tissues (KC)) was introduced. Brownish discoloration was observed on all test-substance treated tissues after the washing procedure. However, in a pretest, it was demonstrated that the color of the test substance did not interfere with the colorimetric test.
The final mean viability of the tissues treated with the test substance determined after an exposure period of 1 hour with an about 42-hour post-incubation was 82.6%.
Based on the results observed, it was concluded that the test item does not show a skin irritation potential in the EpiDerm in vitro skin irritation and corrosion test strategy under the test conditions chosen (BASF, 2017).
Eye irritation/corrosion
The eye irritation and corrosion potential of the test substance was assessed in an in vitro test strategy. Two in vitro assays were part of this strategy: The Bovine Corneal Opacity and Permeability Test (OECD TG 437) and EpiOcular Eye Irritation Test (OECD TG 492). However, the results derived with EpiOcular alone were sufficient for a final assessment. Therefore, further testing in BCOP was waived.
In the study according OECD TG 492, the test item was applied using ca. 50 μL bulk volume (about 30 mg) of the undiluted test substance to a reconstructed three-dimensional, human cornea model (EpiOcular). Two EpiOcular tissues were incubated with the test substance for 6 hours followed by an18 hour post-incubation period. Tissue destruction was determined by measuring the metabolic activity of the tissue after exposure/post-incubation by using a colorimetric test. The reduction of mitochondrial dehydrogenase activity measured by reduced formazan production after incubation with a tetrazolium salt (MTT) was chosen as endpoint. The formazan production of the epidermal tissues treated with the test substance is compared to that of negative control tissues. The ratio of the values indicates the relative tissue viability.
Due to the color of the test substance, it could not be determined whether the test substance is able to directly reduce MTT. Therefore, an additional MTT reduction control (freeze-killed control tissues (KC)) was introduced. In a pretest, it was demonstrated that the color of the test substance interferes with the colorimetric test. Therefore, color controls (viable tissues CC) and freeze-killed control tissues (KC CC) were performed to differentiate formazan produced by the cells in the MTT test from color residues of the test substance in the colorimetric test.
Orange discoloration and minimal compound residues were observed on all test-substance treated tissues after the washing procedure. The values of the color control (CC) tissues indicate interference due to the color of the test substance (mean value 7.6% of NC). The results of the KC tissues indicate an increased MTT reduction also (mean value 1.2% of NC). Thus, for the test substance the final mean viability is given after CC and KC correction. The final mean viability of the tissues treated with the test substance was 70%.
Based on the results observed in the EpiOcular Test alone and by applying the evaluation criteria, it was concluded that the test item does not show an eye irritation potential in the in vitro eye irritation test strategy under the test conditions chosen (BASF, 2017).
Justification for classification or non-classification
Classification, Labeling, and Packaging Regulation (EC) No 1272/2008
The available experimental test data are reliable and suitable for the purpose of classification under Regulation (EC) No 1272/2008. Based on these information the test item is not considered to be classified for skin and eye irritation under Regulation (EC) No 1272/2008, as amended for the tenth time in Regulation (EU) No 2017/776.
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