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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1973
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
other: Method A-11
Deviations:
not specified
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Test organisms (species):
other: Rainbow trout
Details on test organisms:
Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen

Further standard conditions:
- Basins have been cleaned with RBS-35 solution (FLUKA 83462) and rinsed with clean water.
- Testing water should be kept at a pH between 6 and 8.5. In case of deviations, pH needs to be adjusted with NaOH or HCl solution.
- Acclimatization period: minimum 12 days at 10-13 °C for trout.
- Feeding periods: daily (TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany)
- Day-night cycles: 12/12h
- Termination: For termination of the experiment MS-222 (Sandoz) should be used at 1% (Methacain).
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
Not available
Test temperature:
15 °C
pH:
8.1-8.4
Dissolved oxygen:
None
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal concentrations: 100 / 200 and 1000 mg/L
Details on test conditions:
None
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
other: TL0
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
other: TL50
Effect conc.:
450 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
other: TL100
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
None
Results with reference substance (positive control):
No reference substance tested.
Reported statistics and error estimates:
None
Validity criteria fulfilled:
yes
Conclusions:
The TL50 of FAT 36038/A in this fish acute toxicity test was determined to be 450 mg/L.
Executive summary:

The acute fish toxicity test was performed according to equivalent or similar guideline as OECD 203. Rainbow trout were used in this test.

Fishes were tested at these different following concentrations: 100, 200 and 1000 mg/L as in the pre-test all fishes survived at 100 mg/L.

Results:

At 100 mg/L: all fishes survived (pH 8.2 after 48 hours eposure)

At 200 mg/L: all fishes survived (pH 8.1 after 48 hours eposure)

At 1000 mg/L: all fishes died after 24 hours exposure time (pH 8.4 after 24 hours eposure)

In conclusion:

TL0 = 200 mg/L

TL50 = 450 mg/L

TL100 = 1000 mg/L.

The TL50 of FAT 36038/A in this fish acute toxicity test was determined to be 450 mg/L.

Description of key information

The LC50 of FAT 36038/C in an acute fish toxicity test was determined to be 450 mg/L. 

Key value for chemical safety assessment

LC50 for freshwater fish:
450 mg/L

Additional information

In a key acute fish toxicity test performed following a guideline equivalent or similar to OECD test guideline 203, Rainbow trouts were exposed to FAT 36038 for 48-hour to determine the LC values. The TL50 in the acute fish toxicity test was determined to be greater than 450 mg/L after 48 hours exposure time. In two other support studies, Rainbow trouts were exposed to FAT 36038 for 48 hours to determine the acute toxicity following a guideline equivalent or similar to OECD test guideline 203. The 48-hour TL50 of FAT 36038 in these fish acute toxicity test was determined to be >1000 mg /L. Based on the data from these studies the 48-hour TL50 of FAT 36038 to fish can be considered to be 450 mg/L.