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EC number: 208-253-0
CAS number: 518-47-8
Based on mortality of test organism Rainbow trout, Channel catfish and Bluegill, the 24, 48 and 96 hrs LC50 values was given-Rainbow trout: LC50 values at24 hrs - 4198 mg/l48 hrs - 3420 mg/l96 hrs –1372 mg/lChannel catfish: LC50 values at24 hrs -3828 mg/l48 hrs -2826 mg/l96 hrs –2267 mg/lBluegill: LC50 values at24 hrs -5000 mg/l48 hrs -4898 mg/l96 hrs –3433 mg/l
Various studies from peer reviewed journals
for the target chemical Fluorescein sodium (Cas no. 518-47-8) were
reviewed to summarize the following information:
1.The Progressive Fish – Culturist, Vol. 31
(3), Pg. no. 139-142, 1969:
Short term toxicity study to 3
different species of fish was carried out for 96 hrs.The test organism
1. Rainbow trout (Salmo gairdneri)
2. Channel catfish (Ictalurus
3. Bluegill (Lepomis macrochirus)
The dye fluorescein sodium
(9-(0-carboxyphenyl)-6-hydroxy-3-isoxanthenone) was acquired from
Matheson, Coleman, and Bell in a dry powder in a grade.The dye for the
toxicity study was weighed separately for each vessel.Stock solutions of
the water-soluble dye was prepared for concentrations of 5 and 100
p.p.m. in the antimycin-dye mixtures. Because antimycin is relatively
insoluble in water, it was first dissolved in acetone and then diluted
with water.The conc. of test chemical used for the study was 5 and 100
mg/l (5 and 100 ppm), respectively.
The toxicity study was performed at
temperature of 21°C. LC50 value was determined at 24, 48 and 96
hrs.Based on mortality of test organism Rainbow trout, Channel catfish
and Bluegill, the 24, 48 and 96 hrs LC50 values was given-
24 hrs - 4198 mg/l
48 hrs - 3420 mg/l
96 hrs –1372 mg/l
Channel catfish:LC50 values at
24 hrs -3828 mg/l
48 hrs -2826 mg/l
96 hrs –2267 mg/l
24 hrs -5000 mg/l
48 hrs -4898 mg/l
96 hrs –3433 mg/l
2. The Journal of Toxicological Sciences,
Vol. 3, Pg. no. 205-214, 1978:
Short term toxicity study toOryzias
latipeswas carried out for 48 hrs and survival rate (in %) was
determined. As no mortality of test animal was observed after 48 hrs,
the LC0 value was found to be 3,000 mg/l. Thus, the survival rate of
fish after 48 hrs was found to be 100%.
3. The Journal of Toxicological Sciences,
Vol. 3, Pg. no. 205-214, 1978 and 1979:
Short term toxicity study toOryzias
latipeswas carried out for 48 hrs and TLm (median
tolerance limit) value was determined. The TLm value was found to
be 3,000 mg/l at 48 hrs.
tolerance limit) value consider to be median lethal concentration
(LC50) thus based on mortality of test fishes, the 48 hr LC50 value was
found to be 3,000 mg/l.
4. Environmental Monitoring and Assessment,
Volume 38, Issue 1, pp 75-96; 1995:
Ecological assessments for both acute and
chronic effects for the 3 life forms of fish was conduct.During
experimentThe Median Lethal Concentration which is estimated to
be lethal to 50% of fish in 96 hours exposure to the test chemical
(LC50) was found to be 2200mg/l.
5. Hydrogeology Journal (2001) 9:321–325:
Ecotoxicological assessments were based on
the determination of acute toxicity to fish according to DIN 38412, Part
31 (Deutsches Institut für Normung e.V. 1989). For the fish test, zebra
fish were used as a modification of the guideline.
The Median Lethal Concentration which is
found to be lethal to 0% of zebrafish in a 96 hours exposure to the test
chemical (LC0) was found to be greater than 10mg/l
The fluorescent dyes that showed no effect
upon either the genotoxicity or the ecotoxicity tests were classified by
the Working Group as SAFE for use in water tracing thus Uranine is
classified as safe for use in water tracing.
Thus on the basis of the above all
experimental results from peer reviewed journals concluded that the
target chemical Fluorescein sodium (Cas no.518-47-8) can be non-
hazardous to fish.
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