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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 25 April to 13 July 2012
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for analysis were taken at 0 and 24 hours (freshly prepared media), and 24 and 48 hours (old test media).
- Details on test solutions:
- Due to the complex and insoluble nature of the test substance (UVCB), a Water Accommodated Fraction (WAF) approach was considered to be the most suitable method of test media preparation.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: daphnid
- Source: continuously bred in the laboratory
- Age at study initiation (mean and range, SD): less than 24-hour old
- Method of breeding: breeding conditions same as test conditions
- Feeding during test: no
ACCLIMATION
- Acclimation conditions (same as test or not): breeding conditions same as test conditions
- Type and amount of food: Chlorella vulgaris daily and and seaweed during holding - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 19.2 - 21.9 °C (test media)
- pH:
- 7.66 - 8.37
- Dissolved oxygen:
- at least 8.15 mg/L
- Nominal and measured concentrations:
- Nominal loading rates of 0, 1.0, 2.2, 4.6, 10, 22, 46 and 100 mg CAE/L in ASTM medium.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass dishes containing 150 mL of test medium
- No. of organisms per vessel: 5 daphnids
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
OTHER TEST CONDITIONS
- Photoperiod: light cycle of 16 hours
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobilization was observed by visual control after 24 and 48 hours of exposure. - Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 0.68 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 3.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Reported statistics and error estimates:
- The EL50 and EC50 values were calculated using the Spearman-Kärber method.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the conditions of the test, the nominal loading rate of CAE at which no significant (≤ 10%) dose related immobility of Daphnia magna occurred was 1.0 mg/L. The NOELR was therefore considered to be 1.0 mg/L (0.68 mg/L based on estimated CAE concentrations).
Based on nominal loading rates, the 48-hour EL50 value of CAE to Daphnia magna was determined to be 3.7 mg/L (2.5 mg/L based on estimated CAE concentrations). - Executive summary:
In a semi-static acute toxicity test performed according to OECD guideline No.202, daphnids (Daphnia magna) were exposed for 48 hours to nominal loading rates ranging between 1.0 and 100 mg CAE/L in ASTM medium. A control group was also included.Four replicate test and control vessels were prepared (5 daphnids/test vessel). Due to the complex and insoluble nature of the test substance (UVCB), a Water Accommodated Fraction (WAF) approach was considered to be the most suitable method of test media preparation.
Samples of test media were analysed for Total Organic Carbon (TOC), as a measure of the dissolved test substance concentration in new and old test media. Given that TOC analysis is not compound specific, the results represent only an estimation of test substance concentration. Therefore the endpoints were based on both nominal loading rates and estimated CAE concentrations.
After 48 hours of exposure, no immobilization was observed in the control and in the nominal loading rate of 1.0 mg/L. The percentages of immobilisation were 20% and 60% at the nominal loading rates of 2.2 mg/L and 4.6 mg/L, respectively. The percentage of immobilisation was 100% from 10 mg/L to 100 mg/L.
Nominal loading rate (mg/L)
Estimated CAE concentration (mg/L)
Exposure
EL50
NOELR
EC50
NOEC
24 h
22.4
4.6
19.2
3.2
48 h
3.7
1.0
2.5
0.68
Reference
The percentage of immobilization after 24 hours and 48 hours of exposure are presented in the table below
Table 1 Immobilization of daphnids exposed for 48 hours to WAFs of CAE
Nominal loading rate (mg/L) |
Nb of daphnids |
Immobilization at |
|||
24 hours |
48 hours |
||||
Nb. |
% |
Nb. |
% |
||
0 |
4*5 |
0 |
0 |
0 |
0 |
1.0 |
4*5 |
0 |
0 |
0 |
0 |
2.2 |
4*5 |
0 |
0 |
4 |
20 |
4.6 |
4*5 |
0 |
0 |
12 |
60 |
10 |
4*5 |
7 |
35 |
20 |
100 |
22 |
4*5 |
11 |
55 |
20 |
100 |
46 |
4*5 |
11 |
55 |
20 |
100 |
100 |
4*5 |
20 |
100 |
20 |
100 |
Description of key information
Based on nominal loading rates, the 48-hour EL50 value of CAE to Daphnia magna was determined to be 3.7 mg/L (2.5 mg/L based on estimated CAE concentrations).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 3.7 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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