Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989

Materials and methods

Principles of method if other than guideline:
No guideline mentioned .
Method: other
GLP compliance:
no
Type of assay:
micronucleus assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether
EC Number:
200-076-7
EC Name:
2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether
Cas Number:
51-03-6
Molecular formula:
C19H30O5
IUPAC Name:
2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether

Test animals

Species:
mouse
Strain:
Swiss
Sex:
male
Details on test animals or test system and environmental conditions:

Body weight average 30 g at the study initiation
Control animals: Distilled water 5 animals/sex

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
Negative: Vehicle (Distilled water)
Positive: Triethanolamine 0.25 mg/kg b.w.
Frequency of treatment:
2 application, 24 h interval
Post exposure period:
6 h after second treatment
Doses / concentrations
Remarks:
Doses / Concentrations:
), 300, 1000, 3000 mg/kg b.w. was administered in two treatments with an interval of 24 h
Basis:

No. of animals per sex per dose:
60 males, 5 animals/sex/dose
Control animals:
yes
Positive control(s):
Positive: Triethanolamine 0.25 mg/kg b.w.

Results and discussion

Test results
Sex:
male
Genotoxicity:
negative
Toxicity:
not specified

Any other information on results incl. tables

Genotoxicity :

The mean micronucleated cell count for all dose groups were essentially comparable with the concurrent vehicle control group.The positive control, triethanolamine induced a significant genotoxic response.

Applicant's summary and conclusion

Conclusions:
Interpretation of results: negative
There was no increase in the number of micronuclei in treated animals as compared to controls.
Piperonyl Butoxide was not genotoxic in the mouse micronucleus test in vivo.