3- Hydroxy-4-methyoxypyrdine-2-carboxylic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)

Deviations:

yes

Remarks:

The pH of the control and test substance treatment solutions ranged from 7.6 – 8.4 during the exposure. This deviation is not expected to have had an adverse impact on the study.

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxy-4-methoxypyridine-2-carboxylic acid

Cas Number:

210300-09-7

Molecular formula:

C7H7NO4

IUPAC Name:

3-hydroxy-4-methoxypyridine-2-carboxylic acid

Specific details on test material used for the study:

Substance name: X696476
Lot #: XS9-109113-80 (TSN300901)
Purity: 100%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

148 mg CaCO3/L

Test temperature:

12.0 to 12.7°C

pH:

7.6 to 8.4

Dissolved oxygen:

Dissolved Oxygen (new): 10.3 to 10.5 mg/L (100 to 102% sat)
Dissolved Oxygen (old): 6.9 to 8.2 mg/L (67 to 80% sat)

Salinity:

Alkalinity: 160 mg CaCO3/L

Conductivity:

350 μS

Nominal and measured concentrations:

Nominal Concentrations: 0 (control), 0.63, 1.5, 2.5, 5.0, and 10 mg/L
Mean Measured Concentrations:

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

9.21 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

other: lack of sublethal effects

Remarks on result:

other: highest concentration tested

Effect concentrations 2

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 9.21 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: highest concentration tested

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Rainbow Trout 96 h LC50: >9.21 mg/L
Rainbow Trout 96 h NOEC: 9.21 mg/L

Executive summary:

A 96-hour static-renewal test was performed according to OECD 203 with nominal test concentrations of 0 (control), 0.63, 1.5, 2.5, 5.0, and 10 mg/L. Test fish were impartially assigned to treatments by adding one fish per chamber, proceeding from the control, then low to high test substance treatments, and repeating steps as necessary until ten fish were present in each test chamber. The treatments were not replicated. Fish were transferred to new solutions after 48 hours.

Observations for mortality and sublethal responses were made at 24, 48, 72, and 96 hours. Temperature, pH, and dissolved oxygen concentration were measured in each test chamber daily. In addition, a continuous record of the temperature from the water bath was maintained. Alkalinity, hardness, and conductivity were measured in a sample of the dilution water at test initiation.

There was no mortality among control animals during the course of the study. Therefore, control animals satisfied test acceptability criteria for survival (i.e., ≥ 90% or one fish) as stated in the study protocol and the OECD 203 testing guidelines.

Based on mean measured concentrations, the estimated 24-, 48-, 72-, and 96-hour LC50 value was >9.21 mg/L, the highest concentration tested. The slope of the 96-hour concentration-response line could not be calculated. The 96-hour NOEC was 9.21 mg/L, based on mean measured concentrations and a lack of statistically significant mortality and sublethal effects at this, the highest test substance concentration.