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EC number: 814-965-8 | CAS number: 22094-84-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 to 20 July 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Temperature range in the incubator recorded during the test was 19.6 - 20.9°C, 0.3°C above the recommended variation limit.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Materia, Inc.; RP367_ONB-D
- Expiration date of the lot/batch: Retest date 9 August 2018
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient (15±10°C), in the dark
- Stability under test conditions: Not known
- Solubility and stability of the test substance in the solvent/vehicle: Not known
OTHER SPECIFICS:
Purity/Composition: ONB Isomers (2) 93.26%; TCPD Isomers (3 major) 3.92%; Other individual impurities < 0.99% ea; (Taken from Certificate of Analysis)
Chemex reference: ECO 180212
Appearance (observed): Colourless liquid
Solubility (specified): <0.001g/L
Homogeneity (observed): Homogeneous
Date of receipt: 15 February 2018 - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Nominal test concentrations: 0 (control) and 100mg/l; Initial measured test concentrations: 0 (control) and 4.562mg/l
- Sampling method: Chemex SOP E201 based on the OECD 202 guideline Daphnia sp. Acute Immobilisation Test.
- Sample storage conditions before analysis: Ambient (15±10°C), in the dark - Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Not provided
- Source: From cultures maintained at Chemex Environmental International Ltd since July 2014 and originally sourced from a clone of genotype 5.
- Age of parental stock (mean and range, SD): Not provided
- Feeding during test: The Daphnia in the test were not fed.
- Food type: Not provided
- Amount: Not provided
- Frequency: Not provided
- Date of gravid isolation: 18 July 2018
- Age of Daphnia at start of test: ≤ 24 hours.
- Culture conditions: Cultured under static conditions
- Temperature: 20 ± 2°C
- Dissolved oxygen: >60% (or 3mg/l) ASV
- Photoperiod: 16 hours light and 8 hours dark
- Feeding regime: Each working day, a suspension of Chlorella vulgaris. The Daphnia in the test were not fed. - Test type:
- static
- Water media type:
- saltwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Test duration 48+/-1 hour
- Test temperature:
- Temperature range within incubator throughout test: 19.6 - 20.9°C
- pH:
- Initial pH at 0 Hours: 6.92
pH range in control and test concentrations throughout test: 6.81 - 7.46 - Dissolved oxygen:
- Initial Dissolved Oxygen at 0 Hours: 6.98mg/l
DO range in control and test concentrations throughout test: 6.08 - 7.13mg/l - Nominal and measured concentrations:
- Nominal test concentrations: 0 (control) and 100mg/l
Initial measured test concentrations: 0 (control) and 4.562mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: 40ml glass dishes. To limit aerial contamination of the test vessels and reduce evaporative losses, the vessels were covered with a perspex sheet.
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: Test volume: 25ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 reconstituted Daphnia water
- Intervals of water quality measurement: The pH (to 0.01), dissolved oxygen mg/l (to 0.01) and temperature (to 0.1°C) measurements were taken at 0 hours on remaining test solution, and at 48-hour endpoint on pooled replicates for each test and control solution. Temperature of incubation was recorded at 0, 24 and 48 hours.
OTHER TEST CONDITIONS
- Light intensity: Illumination: 16 Hours Light, 8 Hours Dark Cycle
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The number of immobilised Daphnia was recorded after 24 and 48-hour exposure periods. Where possible, the EC(r)10, EC(r)50 EC(r)90 values with 95% confidence limits and NOEC(r) were calculated according to the statistical methods of ToxCalc™ Version 5.0 “Comprehensive Toxicity Data Analysis and Database Software”, copyright 1994-1996.
VEHICLE CONTROL PERFORMED: No
RANGE-FINDING STUDY
- Test concentrations: 0 (control), 0.1, 1, 10 and 100mg/l.
- Results used to determine the conditions for the definitive study: 48-hour EC50 >100mg/l. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (Study in 2017).
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 4.562 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 4.562 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 4.562 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 4.562 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 4.562 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 4.562 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities:
- Observations on body length and weight: No details provided
- Other biological observations: None
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: The Daphnia were examined at 24-hours and 48-hours for abnormal behaviour during the determination of immobility. All Daphnia appeared normal with no signs of abnormality.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: None
No test concentration immobilised all twenty Daphnia after 48-hours. The highest concentration where no immobilisation occurred was 4.562mg/l. - Results with reference substance (positive control):
- A separate GLP study (ENV 11395) was conducted from 18 to 20 January 2017 to monitor the sensitivity of the test animals and test procedures. The reference study gave a 24 hour EC50 of 1.4mg/l of potassium dichromate which was within the acceptable range 0.6 mg/l to 2.1 mg/l as specified in Technical Corrigendum to ISO6341.
- Validity criteria fulfilled:
- yes
- Conclusions:
- 48h EC50 >4.562mg/l (Confidence limits not possible to determine).
48h NOEC >4.562mg/l
48h LOEC >4.562mg/l
Reference
Analytical measurement of the freshly prepared exposure solutions showed that the mean measured concentrations were not within 20% of nominal concentrations. However, analytical measurement of the aged exposure solutions showed that all of the concentrations were within 20% of the initial measured concentrations. Therefore in accordance with OECD Guidelines, the initial measured concentrations have been used in the determination of the EC50, NOEC and LOEC values.
Description of key information
Analytical measurement of the freshly prepared exposure solutions showed that the mean measured concentrations were not within 20% of nominal concentrations. However, analytical measurement of the aged exposure solutions showed that all of the concentrations were within 20% of the initial measured concentrations. Therefore in accordance with OECD Guidelines, the initial measured concentrations have been used in the determination of the EC50, NOEC and LOEC values.
Key value for chemical safety assessment
Marine water invertebrates
Marine water invertebrates
- Effect concentration:
- 4.562 mg/L
Additional information
EC50 >4.562mg/l
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