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EC number: 812-549-0 | CAS number: 881539-89-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Feb - 01 Mar 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Rheinland-Pfalz, Mainz, Germany
Test material
- Reference substance name:
- disodium 4-[(1-hydroxyprop-2-en-1-yl)amino]-4'-(prop-2-enamido)-[1,1'-biphenyl]-2,2'-disulfonate
- EC Number:
- 812-549-0
- Cas Number:
- 881539-89-5
- Molecular formula:
- C18H14N2Na2O8S2
- IUPAC Name:
- disodium 4-[(1-hydroxyprop-2-en-1-yl)amino]-4'-(prop-2-enamido)-[1,1'-biphenyl]-2,2'-disulfonate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Blank control and 100 mg/L (at 0 and 48 h)
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A solution containing 102.4 mg/L in dilution water was prepared.
- Controls: dilution water only
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): 0 - 18 h
- Method of breeding: similar to the method described in the OECD guideline: vessels: preserving glasses (nominal volume 2 L), medium: M4-Medium (recipe of ELENDT), food: green algae (Desmodesmus subspicatus), medium renewal: twice a week, photo period: 16/8 hours (using neon tubes), temperature 20 ± 2 °C
- Source: Umweltbundesamt Berlin (27. September 2007)
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS:
19 hours before the start of the test, the adult animals were separated from the young. 1 hour before test start, adults were caught with the help of a glass tube, and the newborn daphnia, aged between 0 and 18 hours, were sieved from the medium and immediately placed into a beaker containing dilution water. After the settling-in period, animals which showed no apparent damage were used for the test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.50 mmol/L, 250 CaCO3/L
- Test temperature:
- 19.8 - 21.3°C
- pH:
- 7.7 - 7.8
- Dissolved oxygen:
- 8.6 - 9.0 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 100 mg/L
Measured concentrations initial (t-=0) /test end (t-72h): < LOQ, 106.03/111.90 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers, nominal volume 50 mL, tall shape
- Volume of solution: 20 ± 5 mL
- Aeration: no
- Renewal rate of test solution: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO test water according to OECD 202
- Culture medium different from test medium: yes, switching from M4-medium (husbandry) to ISO test water has been shown not to cause any detrimental effects for test daphnia.
- Intervals of water quality measurement: pH and dissolved oxygen (0, 48 h)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h light:dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilised daphnia (24, 48 h) - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: only one of the daphnids was immobilised which can be stated as not significant.
- Results with reference substance (positive control):
- 24h-EC50: 1.9 mg/L (within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L as specified in the OECD guideline)
Any other information on results incl. tables
Table 1: Calculated Test item Concentrations
Nominal Concentration Test Item |
Measured Concentration Test Item t = 0 h |
Measured Concentration Test Item t = 48 h |
% of Nominal concentration t = 0 h |
% of Nominal concentration t = 48 h |
mg/L |
mg/L |
mg/L |
% |
% |
Blank control |
< LOQ |
< LOQ |
-- |
-- |
100 |
106.0 |
111.9 |
106 |
112 |
LOQ (Limit of quantification) = 4 mg/L
Table 2: Immobility
Nominal Concentration in mg/L |
Immobility 24 hours |
Immobility 48 hours |
||||||||
absolute |
in % |
absolute |
in % |
|||||||
Blank control |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
5 |
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Table 3: Validity criteria for OECD 202.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
5% |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
≥ 8.6 mg/L |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
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