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EC number: 700-253-3 | CAS number: 115258-10-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-10-2 to 2010-10-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study according to international Guidelines, fully GLP monitored, no deviations
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N-(3-{1,1,1,5,5,5-hexamethyl-3-[(trimethylsilyl)oxy]trisiloxan-3-yl}propyl)prop-2-enamide
- EC Number:
- 700-253-3
- Cas Number:
- 115258-10-1
- Molecular formula:
- C15H37NO4Si4
- IUPAC Name:
- N-(3-{1,1,1,5,5,5-hexamethyl-3-[(trimethylsilyl)oxy]trisiloxan-3-yl}propyl)prop-2-enamide
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 29, 9.2, 2.9, 0.92 and 0.29 µg test item/L
- Sampling method: no data
- Sample storage conditions before analysis: The freshly prepared test media were stored in a freezer (<= -10°C), protected from light until analysis was performed, whereas the aged test media were measured directly on the day of sampling
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- REPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:acc. to OECD 23 with solvent
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Dimethylformamide
- Concentration of vehicle in test medium (stock solution and final test solution): stock soluition: 290 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain:No. 61.81 SAG
- Source (laboratory, culture collection): SAG
- Age of inoculum (at test initiation):
- Method of cultivation: continous standard culture in exponontial growth
ACCLIMATION
- Acclimation period: not applicable
- Culturing media and conditions (same as test or not): no data
- Any deformed or abnormal cells observed: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- no
Test conditions
- Hardness:
- OECD medium
hardness (calculated): 0,24 mmol/L ^= 24 mg/L as CaO) - Test temperature:
- 23-24
- pH:
- 8.0 (start); 8.9 - 9.5 (test end)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmayer flask
- Type (delete if not applicable): open but covered
- Material, size, headspace, fill volume: glass, 50 ml
- Initial cells density: 5000 cells/mL
- Control end cells density: 72.745E+5
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used: not applicable
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD water
- Culture medium different from test medium: no data
- Intervals of water quality measurement: no data
OTHER TEST CONDITIONS
- Sterile test conditions: no data
- Adjustment of pH: no data
- Photoperiod: 24/0
- Light intensity and quality:6035 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometer
- Chlorophyll measurement: no
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3,15
- Justification for using less concentrations than requested by guideline:
- Range finding study yes
- Test concentrations: 0.29, 0.92, 2.9, 9.2, 29 µg/L (each in 100 µL DMF/L)
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 29 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 29 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 29 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 29 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 29 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 29 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
- Unusual cell shape: no
- Colour differences: no data
- Flocculation: no
- Adherence to test vessels: not reported
- Aggregation of algal cells: not reported
- Other:
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: yes - Reported statistics and error estimates:
- not applicable, due to no observed effect in the tests
Any other information on results incl. tables
I Biological results
Parameter |
Growth rate |
Yield |
72-hour EC50 |
> 29 |
> 29 |
95 % conf. limits |
n.d. |
n.d. |
|
|
|
72-hour EC10 |
> 29 |
> 29 |
95 % conf. limits |
n.d. |
n.d. |
|
|
|
72-hour NOEC |
≥ 29 |
≥ 29 |
72-hour LOEC |
> 29 |
> 29 |
n.d. = not determinable |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under test conditions no inhibition of growth, rate and yield could be observed. The test item is regarded as not toxic up to the water solibility of 29 µg/L to the tested green algae Pseudokirchneriella subcapitata.
- Executive summary:
The influence of N-[3-tris(trimethylsilyloxy) silylpropyl]prop-2-enamide on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static dose-response test. The 72-hour NOEC was determined to be at least 29 µg test item/L. The NOEC might even be higher than this concentration, but concentrations in excess of the limit of solubility have not been tested according to the recommendations of the test guidelines. The 72-hour LOEC and the 72-hour EC50were clearly higher than 29 µg test item/L. These values could not be quantified due to the absence of toxicity of N-[3-tris(trimethylsilyloxy) silylpropyl]prop-2-enamide up to the limit of solubility.
Under test conditions no inhibition of the test item to the tested algae were detectable.
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