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EC number: 700-183-3 | CAS number: 52299-25-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-09-20 - 2010-11-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study was performed in compliance with the Good Laboratory Practice (GLP) regulations (revised in 1997, ENV/MC/CHEM(98)17). The method followed that described in the OECD Guidelines for Testing of Chemicals (Adopted: 4 April 1984) No 202 "Daphnia sp., Acute Immobilisation Test. and Reproduction Test".
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- None
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- bis(nonafluorobutyl)phosphinic acid
- EC Number:
- 700-183-3
- Cas Number:
- 52299-25-9
- Molecular formula:
- C8HF18O2P
- IUPAC Name:
- bis(nonafluorobutyl)phosphinic acid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- The stability of the test material in the test medium under the conditions of the study was shown in a separate analytical study prior to start of this study. Due to the absence of any adverse effects no analytical sampling was performed during the study.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Reconstituted water according to ELENDT (1990):
Macro nutrients (mg/L):
CaCI2 x 7 H20 293.80
MgSO4 x 7 H20 123.30
NaHCO3 64.80
KCI 5.80
Na2SiO3 x 9H20 10.00
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18
Trace elements (mg/L):
B 0.5000
Fe 0.2000
Mn 0.1000
Li, Rb and Sr 0.0500
Mo 0.0250
Br 0.0125
Cu and Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003
Macro nutrients (mg/L):
Na2EDTA x 2H20 2.50
Vitamins (µg/L):
Thiamine 75.00
B12 1.00
Biotin 0.75
After preparation, the reconstituded water was aerated for 24 hours.
- Hardness: about 14° dH (about 250 mg/L CaCO3)
- pH: 7.9 ± 0.3, after an aeration for 24 hours.
References:
ELENDT, B.-P. Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus.
Protoplasma 154, 25-33, 1990
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 14 ° dH (about 250 mg/L CaCO3)
- Test temperature:
- 22°C
- pH:
- 7.5 +/- 0.3
- Dissolved oxygen:
- >3 mg/L
- Nominal and measured concentrations:
- Nominal 100 mg/L
- Details on test conditions:
- -- Preparation
The test medium (reconstituted water and test material) was freshly prepared. Therefore, the calibrated flask with test material and vehicle, reconstituted water, was treated in an ultrasonic device for 1 hour. Subsequently the preparation was aerated, and stirred with a magnetic stirrer for further 23 hours.
-- Administration
At the start of the experimental phase, 5 Daphnia magna were placed into 10 ml of reconstituted water (control group) or test medium (test material group). The Daphnia magna were not fed, and the control medium and test medium were not aerated during the test.
The test vessels were labeled to assure an unmistakable identification.
-- Dose levels
A single test material concentration of 0.1 g/L was tested in an open static system.
-- Observation schedule
The mobility was determined by visual control and recorded after 24 and 48 hours.
-- pH and oxygen concentration
The pH-values and dissolved oxygen concentration were measured in the control and all test material concentrations at the beginning and at the end of the experimental part.
-- Temperature
During the experimental part, the temperature was registered in a control vessel with an electronic thermometer containing a maximum and minimum memory display.
-- Archive statement
All raw data, specimens and the final report are stored in the archives of the test facility. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- A nominal test concentration of 100 mg/L revealed no aquatic toxicity in this test system.
Any other information on results incl. tables
The concentration of 100 mg/L revealed no biological effects (EC0).
Due to the absence of toxic effects at the limit concentration, EC50 values cannot be calculated.
24 h EC50 >100 mg/L
48 h EC50 >100 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item solved in reconstituted water, was tested in an open static test system. Under the given conditions of this study the 48 h EC50 value was >100 mg/L.
- Executive summary:
Purpose
The purpose of this assay was to identify the aquatic toxicity potential of the test material in Daphnia magna to provide a rational basis for hazard estimation for the test item in aquatic environments.
Study design
For this purpose, juvenile Daphnia magna were exposed to an aqueous test material preparation over 48 hours, under defined conditions. The study comprised of four test vessels per concentration containing five Daphnia magna each, i.e. 20 Daphnia per concentration (test medium group). In addition, a control group of 20 Daphnia in reconstituted water was used.
Daphnia magna were exposed to a test material concentration of nominal 100 mg/L (limit test) in an open static system.
Results
After exposure to a saturated aqueous solution of the nominal concentration of 0.1 g/L for 48 hours to Daphnia magna the following results were obtained:
Nominal concentration Immobilization immobilized / exposed
[g/L] 24 hours 48 hours 0.0 0/20 0/20 0.1 0/20 0/20 Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg/L of the test material were not affected.
The test material concentration in the aqueous medium was not quantified at the start and the end of this study because the stability of the test material in the test medium under the conditions of the study has been shown in a separate analytical study prior to start of this study and due to the fact that no toxicity was observed at the limit concentration (100 mg/L).
For the test material, the following nominal EC50 values for Daphnia magna were determined:
24 h EC50> 100 mg/L
48 h EC50> 100 mg/L
Conclusions
An aqueous solution of 100 mg/L test material revealed no aquatic toxicity in the test system. The 48 hours EC50 to Daphnia magna exceeded the limit concentration (>100 mg/L) and, thus, could not be determined in this test.
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