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Diss Factsheets
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EC number: 605-076-4 | CAS number: 156928-09-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-04-19 to 2007-10-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Remarks:
- The testing facility indicated that th eprotocol was followed without deviation.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- Remarks:
- The testing facility indicated that th eprotocol was followed without deviation.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- The Federal Office of Public Health, the Swiss Agency for Therapeutic Products and the Swiss Agency for the Environment, Forests and Landscape with respect to the compliance with the Swiss legislation on Good Laboratory Practice.
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: ARA Ergolz II, Fϋllinsdotf, Switzerland
- Laboratory culture: not applicable
- Method of cultivation: no data
- Storage conditions:at room temperature with aeration until use
- Storage length: one day prior to use
- Preparation of inoculum for exposure: The sludge was washed three times with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated. Based on this ratio, calculated amounts of wet sludge were suspended in test to obtain a concentration equivalent to 4 g ± 10% dry material per liter. During the holding period of one day prior to use, the sludge was aerated at room temperature. Prior to use, the sludge was first thoroughly mixed and then diluted with test water to a concentration of 1 g per liter (dry weight basis). Based on the determined dry weight of this diluted activated sludge defined amounts were added to test water to obtain a final concentration of 30 mg dry material per liter.
- Pretreatment: no data
- Concentration of sludge: 1 g per liter (dry weight basis)
- Initial cell/biomass concentration: no data
- Water filtered: The test water was prepared according to the testing guidelines
- Type and size of filter used, if any: not applicable - Duration of test (contact time):
- 28 d
- Initial conc.:
- 174 mg/L
- Based on:
- ThOD/L
- Initial conc.:
- 173 mg/L
- Based on:
- ThOD/L
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Initial conc.:
- 101 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: aerobic activated sludge prepared with test water
- Additional substrate: no
- Solubilising agent:none
- Test temperature: 22 °C
- pH: 7.4 - 7.6
- pH adjusted: The pH of the test water was adjusted from 7.8 to 7.4 with a diluted hydrochloric acid solution
- Aeration of dilution water: no data
- Suspended solids concentration: 30 mg dry material per liter
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 500 mL Erlenmeyer flasks
- Number of culture flasks/concentration: two (101 mg/L test substance (ThOD 174 mg O2/L), 100 mg/L test substance (ThOD 173 mg O2/L))
- Method used to create aerobic conditions: the consumed oxygen is replaced by electrolytically generated oxygen from copper sulfate solution
- Method used to create anaerobic conditions: not applicable
- Measuring equipment: electrode type manometer (The biodegradation process consumes the dissolved oxygen in the liquid and generates C02. The C02 is adsorbed by soda lime and the total pressure decreases in the airtight test flasks. The pressure drop is detected and converted into an electrical signal by means of an electrode type manometer)
- Details of trap for CO2 and volatile organics if used: adsorbed by soda lime
SAMPLING
- Sampling frequency: continious measurement
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (in duplicate = two flasks without test substance or reference substance)
- Abiotic sterile control: no
- Toxicity control: yes
- Other: two procedure controls with 100 mg/L reference substance - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- No circumstances that may have affected the results during the test.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1
- Sampling time:
- 28 d
- Remarks on result:
- other: The mean 28 day biodegradation of both replicates was 1%
- Details on results:
- The BOD of the test item T002675 in the test media was in the normal range found for the inoculum controls. T002675 was found to be not biodegradable under the test condition within 28 days.
- Results with reference substance:
- The reference item (sodium benzoate) was degraded by an average of 90% by day 14, and reached an average biodegradation of 91% by the end of the test (day 28), thus confirming suitability of the activated sludge.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- A 28-d ready biodegradability test (OECD 301F) using unadapted activated sludge from a predominantly domestic waste water treatment plant indicated that T002675 was not readily biodegradable under the conditions of the test. The test substance did not inhibit microbial activity at the concentration used in the test. The results of the test can be considered reliable without restriction.
Reference
According to the test guidelines, the test substance had no inhibitory effect on activated sludge microorganisms at the tested concentration of 100 mg/L because biodegradation in the toxicity control was >25% within 14 days.
Description of key information
One study (Seyfried, 2007) is included in this dossier and regarded as a key study (Klimisch score of 1). The biodegradability of T002675 was determined according to OECD Guideline 301F and EU Method C.4-D. Under the conditions of the test, T002675 was determined to be not biodegradable within 28 days.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.