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EC number: 929-915-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study without restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 4-{[(6-{2,4,6-trioxo-3,5-bis[6-({[4-(prop-2-enoyloxy)butoxy]carbonyl}amino)hexyl]-1,3,5-triazinan-1-yl}hexyl)carbamoyl]oxy}butyl prop-2-enoate
- EC Number:
- 929-915-1
- IUPAC Name:
- 4-{[(6-{2,4,6-trioxo-3,5-bis[6-({[4-(prop-2-enoyloxy)butoxy]carbonyl}amino)hexyl]-1,3,5-triazinan-1-yl}hexyl)carbamoyl]oxy}butyl prop-2-enoate
1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The definitive test was performed using the following test item concentrations: filtrates of a loading of:
1000, 333, 111, 37, 12.3, 4.12 mg/L plus the control.
Appropriate amounts of the test item were weighed separately in glass flasks partially filled with the
AAP medium and filled up with appropriate volume of the AAP medium (Table 4) [SOP/W/7].
The mixtures were visually heterogeneous. Therefore, the mixtures and the control (the AAP medium,
1200 mL) were mechanically shaken for 48 h (30ºC, darkness, 90 revolutions per minute). After
48 hours of mechanical shaking, the mixtures (heterogeneous) and the control were stored at room
temperature for a settling phase (no shaking). After 24 hours of settling phase, the mixtures were
visually heterogeneous. Therefore, the mixtures and the control were filtered through a conditioned
nitrocellulose membrane filter (0.22 Vm, Millipore), [SOP/W/37]. This is a WAF-approach followed by
filtering which results in a water soluble fraction [9]. Filtration was chosen as the separation method
since centrifugation is not possible with the large volumes of test solution. Each filtrate was visually
homogeneous and transparent.
At exposure initiation, four samples (without algae) of the filtrate of a loading of 1000 mg/L and the
control were collected [SOP/W/83]. One of them was transferred for chemical analyses at exposure
initiation, whereas the other was stored under test conditions and transferred for chemical analyses
daily. Moreover, at exposure initiation, two samples (without algae) of the filtrates of a loading of 333,
111, 37, 12.3 and 4.12 mg/L were collected [SOP/W/83]. One of them was transferred for chemical
analyses at exposure initiation, whereas the other was stored under test conditions and transferred for
chemical analyses after 72 h of exposure.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: SAG 61.81
- Source (laboratory, culture collection): Algae Collection University of Göttingen, Germany, cultivated at the performing institute.
- Method of cultivation: Algae were transferred from agar bevels to the fresh medium (for Pseudokirchneriella subcapitata AAP medium is recommended, Appendix 2) in 250 mL Erlenmeyer flask and incubated in 21 – 24 C in constant illumination (pre-culture) [1], [SOP/W/65]. The algae culture was renewed (inoculated to a new medium) twice a week in sterile conditions [SOP/W/56, SOP/W/70].
A pre-culture with cell density of 1 x 104 per mL was renewed three days before the definitive test. The pre-culture was used for inoculation of the test concentrations and the control.
ACCLIMATION
- Culturing media and conditions (same as test or not): yes
- Any deformed or abnormal cells observed: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass Erlenmeyer flasks 250 mL
- Type (delete if not applicable): closed with air permeable stoppers
- Material, size, headspace, fill volume: 100 mL
- Aeration: none
- Initial cells density: 10E+04 cells/mL
- Control end cells density:
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 2
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16:8 light:dark
- Light intensity and quality:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometric measurement
TEST CONCENTRATIONS
- Range finding study: yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 698 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence interval: 672 – 723
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 369 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence interval: 340 – 396
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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