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EC number: 286-304-6 | CAS number: 85204-10-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to international guideline(s), GLP-compliant, performed in recognized contract research organization, no restrictions, fully adequate for assessment.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: sewage plant for domestic sewage in 8200 Veszprém, Hungary
- Method of cultivation / Preparation of inoculum for exposure: The activated sludge was washed by centrifugation (for 10 min.) and the supernatant liquid phase was decanted. The solid material was re-suspended in isotonic saline solution and again centrifuged (for 10 min.). This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 4 g dry material per litre were mixed with test water and then aerated until use (not later than an hour). Before use the sludge was filtered through cotton wool. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2.9 mg/L
- Based on:
- test mat.
- Initial conc.:
- ca. 6 mg/L
- Based on:
- COD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral salts medium according to guidelines, prepared using deionised water.
- Test temperature: 21-23 °C
- pH: The pH value of the test water was checked prior study start. The pH of the test water was 7.4.
- Aeration of dilution water: The test medium was aerated for 20 minutes and allowed to stand for about 20 hours at the test temperature. The dissolved oxygen concentration was 8.9 mg/L at about 22 °C.
- Suspended solids concentration: 0.5 mL microbial inoculum/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: BOD bottles (300 mL) with glass stoppers, temperature-controlled incubator
- Number of culture flasks/concentration and sampling date: 2
- Measuring equipment: oxygen meter with a stirring O2 electrode
SAMPLING
- Sampling frequency: Day 0, 7, 14, 21 and 28
- Sampling method: Two bottles from each study group were removed for measurement of the oxygen content.
CONTROL AND BLANK SYSTEM
- Inoculum blank (2 flasks/sampling date): inoculated mineral salts medium (MSM)
- Reference (2 flasks/sampling date): inoculated MSM + 3.6 mg sodium benzoate/L (corresponding to 6.0 mg O2/L)
- Toxicity control (2 flasks/sampling date): inoculated MSM + 2.9 mg test material/L (6.0 O2/L) + 3.6 mg sodium benzoate/L (6.0 mg O2/L) - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- During a 14-day preliminary test, the concentration of dissolved oxygen resulted a mean of 7.40 mg O2/L after 14 days of incubation in the test item flasks, which was nearly at the control level (7.80 mg O2/L). Therefore, measurement of the total oxidised nitrogen (nitrate and nitrite) concentrations was not performed in the main test.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 13
- Sampling time:
- 28 d
- Remarks on result:
- other: mean of duplicates
- Details on results:
- Biodegradation of the test material at test end: 11% and 14% based on COD (Table 1).
In the toxicity control (test item + sodium benzoate), a mean of 36% biodegradation was noted within 14 days and 45% biodegradation after 28 days of incubation indicating that the test substance was not inhibitory to the microbial inoculum (Table 1). - Results with reference substance:
- The reference substance sodium benzoate had achieved 73% of the ThOD after 14 days of incubation and 91% by Day 28 (Table 1), thus confirming the suitability of the used activated sludge inoculum.
- Validity criteria fulfilled:
- yes
- Remarks:
- Difference of extremes of replicate values of the removal of the test substance <20%; degradation of the reference compound ≥60% by 14 days; residual O2 content ≥ 0.5 mg/L; oxygen uptake of the inoculum blank < 1.5 mg/L after 28 days.
- Interpretation of results:
- under test conditions no biodegradation observed
Reference
Table 1 Biodegradation of the test and reference substance
Treatment |
Flask |
Percent of biodegradation after n days of exposure |
|||
|
No. |
7 |
14 |
21 |
28 |
Test item |
a |
3.3 |
4.2 |
10.0 |
10.9 |
b |
1.7 |
5.9 |
10.0 |
14.2 |
|
mean |
2.5 |
5.0 |
10.0 |
12.6 |
|
Reference item |
a |
56.7 |
70.8 |
85.0 |
89.2 |
b |
58.3 |
75.8 |
88.3 |
92.5 |
|
mean |
57.5 |
73.3 |
86.7 |
90.8 |
|
Toxicity control |
a |
28.9 |
35.9 |
43.8 |
45.0 |
b |
28.1 |
36.7 |
44.6 |
45.0 |
|
mean |
28.5 |
36.3 |
44.2 |
45.0 |
Description of key information
Biodegradation in water: screening tests: aerobic biodegradation 13% (COD) in 28 days (OECD 301D, EU C.4-E)
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.