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EC number: 271-678-5 | CAS number: 68603-87-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- Department of the environment, test proposal: Letale Wirkung beim Zebrabaerbling Brachydanio rerio, (LC0, LC 50, LC100, 48-95h), Stand Mai 1984
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Details on test solutions:
- At the beginning of the test the pH is adjusted at each concentration.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- n=10
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 20.8-21.6 °C
- pH:
- 5.7-7.3
- Dissolved oxygen:
- 9.3-5.1 mg/L
- Nominal and measured concentrations:
- 100, 35.4 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 35.4 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 59.5 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: ; geometric mean
- Sublethal observations / clinical signs:
conc. 100 mg/L
Time C° O2 (mg/L) pH mortality (%) Fish with effects / fish alive 0h 21.2 8.4 4.0/6.8 --- 0/10 24h 21.6 8.6 5.7 0 0/10 48h 21.2 6.0 6.6 10 9/9 72h 21.3 7.1 7.3 100 0/0 96h --- --- --- --- --- conc. 35.4 mg/L
Time C° O2 (mg/L) pH mortality (%) Fish with effects / fish alive 0h 20.8 9.3 4.4/6.9 --- 0/10 24h 21.6 8.3 6.2 0 0/10 48h 21.5 5.1 6.7 0 0/10 72h 21.3 7.9 7.1 0 0/10 96h 21.1 8.0 7.1 0 0/10 - Conclusions:
- After 96 hours of exposure a LC50 of 59.5 mg/L (geometric mean) was obtained.
- Executive summary:
The acute toxicity of Carboxylic acids, di-,C4-C6 to fish (Brachydanio rerio) was conducted in a static mortality test similiar to OECD Guideline 203 (Fish, Acute Toxicity Test). After 96 hours of exposure a LC50 of 59.5 mg/L (geometric mean) was obtained.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Principles of method if other than guideline:
- DIN-Standard 38 412 (Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung - Testverfahren mit Wasserorganismen (Gruppe L)) - Bestimmung der Wirkung von Wasserinhaltstoffen auf Fische, Fischtest (L 15), Entwurf 1979
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on test solutions:
- At the beginning of the test, the test solutions are clear at every concentration. At the end of the test the solutions became more opalescent with increasing concentration .
- Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- - source: Firm P. Eggers, Hohenwestedt, West Germany
- No medical treatment during the last four weeks of keeping.
- Acclimation period (keeping water to test water) : 3 days - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- total hardness: 2.6 mmol/L
- Test temperature:
- 20°C (+-1°C)
- pH:
- 8+-0.1
- Dissolved oxygen:
- > 8 mg/L
- Nominal and measured concentrations:
- nominal concentrations were tested (68.1, 100, 147, 215, 316, 464 and 681mg/L).
- Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 147 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 147 - < 215 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Results with reference substance (positive control):
- LC50 (48h) ca. 25 mg/L (correspond to the normal sensitiveness)
- Sublethal observations / clinical signs:
table 1: mortality
Conc. mortality
mg/L n fish (0h) 1 h 4 h 24 h 48 h 72 h 96 h 68.1 10 0 0 0 0 0 0 100 10 0 0 0 0 0 0 147 10 0 0 0 0 0 0 215 10 0 0 * 1 4 8 8 316 10 0 0 * 9 ** 10 10 10 464 10 0 10 10 10 10 10 681 10 1 10 10 10 10 10 Control 10 0 0 0 0 0 0 *= the animals showed the following symptoms: gasping for air, abnormal swimming action
**= the animals showed the following symptoms: abnormal swimming action
table 2: pH values
conc. pH values
mg/L 0 h 24 h 48 h 72 h 96 h 68.1 6.4 5.8 6.7 7.2 7.4 100 6.1 5.1 5.2 5.6 6.2 147 5.9 4.7 4.7 5.2 5.7 215 5.8 4.4 4.3 4.6 5.4 316 5.7 4.1 4.1 --- --- 464 5.5 3.8 --- --- --- 681 5.5 3.8 --- --- --- Control 7.8 7.9 8.0 8.0 7.9 table 3: oxygen values
conc. oxygen values
mg/L 0 h 24 h 48 h 72 h 96 h 68.1 8.6 8.7 8.7 8.9 7.1 100 7.5 8.0 7.2 6.8 5.5 147 7.4 7.5 6.8 3.6 7.1 215 7.7 7.6 7.6 1.1 6.0 316 7.9 8.4 8.9 --- --- 464 8.6 8.9 --- --- --- 681 8.8 9.0 --- --- --- Control 8.9 8.8 8.9 9.0 8.9 Additionally, a test at a concentration of 681 mg/l in neutralised test solution (10% NaoH) was carried out. Exposure duration was not reported. No effects were observed.
- Conclusions:
- After 96 hours of exposure an LC50 >147 <215 mg/L (nominal) was obtained.
- Executive summary:
The acute toxicity of Carboxylic acids, di-,C4-C6 to fish (Leucisus idus) was conducted in a static mortality test according to national standard method (DIN-Standard 38 412). After 96 hours of exposure an LC50 >147 <215 mg/L (nominal) was obtained.
Referenceopen allclose all
Description of key information
The acute toxicity of Carboxylic acids, di-,C4-C6 to fish (Brachydanio rerio) was conducted in a static mortality test similiar to OECD Guideline 203 (Fish, Acute Toxicity Test). After 96 hours of exposure a LC50 of 59.5 mg/L (geometric mean) was obtained (Bayer, 1988).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 59.5 mg/L
Additional information
There are two tests on toxicity to fish available.
The dicarboxylic acids are of weak to medium acidity and partly dissociate in water causing a pH shift (pKa1: 4.21-4.43; pKa2: 5.42-5.64). The tolerance of water organisms towards pH is diverse. Recommended pH values for test species listed in OECD guidelines are between 6 and 9.
In an acute test performed with Leuciscus idus according to the German national standard method DIN 38412 Part 15 a 96 h-LC50 of > 147 mg/l for the mixture with following composition: 24-28% adipic acid, 40-45% glutaric acid, 28-33% succinic acid was obtained (BASF, 1980). At test concentrations above 220 mg/l used in the aquatic toxicity test the pH shifts to <6. At 147 mg/L no effect was observed. At higher concentrations some mortalities occurred, along with reduced pH (below pH 4.5). As effect of pH allone at higher concentrations is unclear, as a precautionary approach the LC50 was assumed to be > 147 mg/L. This assumption is confirmed by a test carried out in neutralised test solution at a concentration of 686 mg/L where no effects were observed.
The acute toxicity of Carboxylic acids, di-,C4-C6 to fish (Brachydanio rerio) was conducted in a static mortality test similiar to OECD Guideline 203 (Fish, Acute Toxicity Test). After 96 hours of exposure a LC50 of 59.5 mg/L (geometric mean) was obtained in buffered medium (Bayer, 1988). The test substance was a residue from adipic acid manufacturing containing the following composition: 60 % adipic acid, 14 % glutaric acid, 6 % succinic acid, 10 % carbon, 6.5 % vanadium pentoxide, 3 % copper nitrate and 5 % copper.
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