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EC number: 262-114-9 | CAS number: 60239-68-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August 09, 2017 to August 16, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of the exposure (0 hours), sampling was carried out after preparation of the test substance concentrations. At the end of the exposure (48 hours), samples of the old media were taken from the test vessel.
- Vehicle:
- no
- Details on test solutions:
- Stock solution: the stock solution of 100 mg/L was prepared with dilution water prior to the start of the exposure (at 0 hours). The stock solution was mixed thoroughly by manual agitation.
Test concentrations: 5 test substance concentrations in a geometric series with a separation factor of 2 were prepared by dilution of the stock solution 100 mg/L with dilution water, as follows: 6.25 - 12.5 - 25.0 - 50.0 - 100 mg/L. The test concentrations are based on the results of a non-GLP preliminary range finding test.
Control: Dilution water without test substance incubated under the same conditions as the test groups. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test system: Daphnia magna STRAUS (Clone 5),
Origin: Institut fur Wasser, Boden und Lufthygiene (WaBoLu), 14195 Berlin, Germany,
Breeder: Noack Laboratorien GmbH, Germany,
Culture: In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 ± 2 °C, in an incubator, 16 hours illumination; light intensity of max. 1500 lx,
Culture medium: Elendt M4, according to OECD 202, Annex 3 (2004), modified to a total hardness of 160 to 180 mg CaCO3/L,
Culture feeding: The culture daphnids were fed at least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus, with an algae cell density of > 10*E06 cells/mL. The algae were cultured at the test facility,
Origin of the food algae: Sammlung von Algenkulturen (SAG), Institut der Universitat Gottingen, Germany. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- total hardness of 160 to 180 mg CaCO3/L
- Test temperature:
- 18 - 22 °C, constant within ± 1°C
- pH:
- 7.45 - 8.08
- Dissolved oxygen:
- from 7.40 to 8.98 mg/L
- Conductivity:
- 444 uS/cm
- Nominal and measured concentrations:
- - Nominal: 6.25, 12.5, 25.0, 50.0, 100 mg/L
- Measured: The measured concentrations of the test substance were in the range of 104 to 119 % of the nominal values at the start of the exposure (0 hours) and 99 to 119 % of the nominal values at the end of the exposure (48 hours). The measured test substance concentrations were all within ± 20 % of the nominal concentrations. - Details on test conditions:
- Test vessels: Glass beakers (4 (ID) x 7 (H) cm), 50 mL capacity, loosely covered with watch glasses.
Test volume: 20 mL.
Number of daphnids and replicates: 20 daphnids, divided into 4 replicates, each with 5 daphnids, were used for each concentration level and the control.
Age of the organisms at the start of exposure: Less than 24 hours old daphnids from a healthy stock. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 48 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (95% CI: 26.4 - 87.2 mg/L)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 45 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: (95% CI: 25.2 - 85.6 mg/L)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- 24 EC50: 1.90 mg/L; 95% CI: 1.43 - 2.69 mg/L.
- Reported statistics and error estimates:
- The EC10/50-values (after 24 and 48 hours of exposure) were calculated by sigmoidal dose-response regression with the software GraphPad Prism. The respective 95 % confidence limits were calculated from the standard error and the t-distribution. All calculations were carried out from the best-fit values with the software GraphPad Prism.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the nominal 48 h EC50 was determined to be 48.0 mg/L (equivalent to 43.5 mg a.i./L) (95% CI: 26.4 — 87.2 mg/L) and the 48 h EC10 was 45.0 mg/L (equivalent to 40.8 mg a.i./L) (95% Cl: 25.2 — 85.6 mg/L).
- Executive summary:
A study was conducted to determine the toxicity of the test substance, C11-unsatd. DEA (90.6% active), to Daphnia magna according to OECD Guideline 202 and EU Method C.2, in compliance with GLP. Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to the test substance (directly diluted in water) in the range of 6.25 - 100 mg/L, prepared in a geometric series with a separation factor of 2 and including a control. Test substance concentrations were analytically verified via LC-MS/MS at the start and end of exposure in all dose groups and in the control. Measured concentrations were in the range of 104 - 119% of nominal values at test start and 99 - 119% of nominal values at test end, therefore results were based on nominal values. Under the study conditions, the nominal 48 h EC50 was determined to be 48.0 mg/L (equivalent to 43.5 mg a.i./L) and the 48 h EC10 was 45.0 mg/L (equivalent to 40.8 mg a.i./L) (Herrmann, 2017).
Reference
None.
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 48 mg/L
Additional information
A study was conducted to determine the toxicity of the test substance, C11-unsatd. DEA (90.6% active), to Daphnia magna according to OECD Guideline 202 and EU Method C.2, in compliance with GLP. Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to the test substance (directly diluted in water) in the range of 6.25 - 100 mg/L, prepared in a geometric series with a separation factor of 2 and including a control. Test substance concentrations were analytically verified via LC-MS/MS at the start and end of exposure in all dose groups and in the control. Measured concentrations were in the range of 104 - 119% of nominal values at test start and 99 - 119% of nominal values at test end, therefore results were based on nominal values. Under the study conditions, the nominal 48 h EC50 was determined to be 48.0 mg/L (equivalent to 43.5 mg a.i./L) and the 48 h EC10 was 45.0 mg/L (equivalent to 40.8 mg a.i./L) (Herrmann, 2017).
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