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EC number: 253-391-7 | CAS number: 37203-76-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-02-26 - 2015-06-11
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ethyl dihydrogen phosphate
- EC Number:
- 216-603-9
- EC Name:
- Ethyl dihydrogen phosphate
- Cas Number:
- 1623-14-9
- Molecular formula:
- C2H7O4P
- IUPAC Name:
- Phosphoric acid,monoethyl ester
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Test item: Hordaphos MDE
CAS number: 37203-76-2
Purity: 98.8 %
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test item concentrations were weighed out directly in Erlenmeyer flasks.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Test System: Activated sludge
Origin: Municipal sewage treatment plant of 31137 Hildesheim, Germany
Reason for the selection: Non-adapted activated sludge from the sewage plant at Hildesheim is well suited as it receives predominantly municipal sewage and hardly any industrial chemical waste.
Pretreatment: The sludge was washed twice with chlorine free tap water and adjusted to a dry sludge concentration of 3.0 g/L ± 10 %. The sludge was used within 24 h after sampling.
Dry sludge concentration: 2.91 g/L, corresponding to 1.46 g/L in the test vessel
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- Nominal: 18-22 °C, actual: 21.0 °C
- Nominal and measured concentrations:
- Nominal test item concentration [mg/L]:10, 32,100,320, 1000, 1000
- Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 375 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- In the reference test the ECso-value for copper (II) sulphate pentahydrate was determined by probit analysis with:
EC5o = 111 mg/L 95%
Confidence Interval = 107-115 mg/L
This is in the recommended range of validity of 53 - 155 mg/L.
Any other information on results incl. tables
Biotic and Abiotic Factors |
pH-value of the activated sludge |
7.75 |
pH-value of the synthetic waste water |
7.38 |
Dry solids concentration [g/L] of sludge |
2.91 |
Dry solid concentration [g/L] in test solution |
1.46 |
Test temperature [°C] |
21.0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of Hordaphos MDE is 100 mg/L. The test item is not toxic up to the concentration of 100 mg/L to activated sludge of a municipal sewage treatment plant. The EC10 is 226 mg/L, the EC50 is 375 mg/L and EC8o is 439 mg/L of the test item.
- Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item Hordaphos MDE (batch number: DEH2 031109) from 2015-03-30 to 2015-03-31 . The test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the test item concentrations 10 - 32 - 100 - 320 - 1000 - 1000 pH mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from 8 % to 97 % . Additionally, replicates with 1000 mg/L and pH-adjustment were prepared. The mean inhibition of the respiration of these replicates was -17 %.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC5o-value for the reference item was 111 mg/L.
The NOEC of Hordaphos MDE is 100 mg/L. The test item is not toxic up to the concentration of 100 mg/L to activated sludge of a municipal sewage treatment plant. The EC10 is 226 mg/L, the EC50 is 375 mg/L and EC8o is 439 mg/L of the test item.
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