Dipotassium titanium oxide dioxalate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019-05-21 to 2019-06-26

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental study performed according to the OECD 202 guideline and under GLP conditions. The validity criteria were successful.
No temperature measurement was recorded during the test because the temperature probe has been accidentally unplugged. There is no impact since validity criteria were met and temperature records in the room were in the good range before and after the test. Therefore, the study can be considered as reliable.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia, Daphnia magna Strauss clone 5, between 6 and 24 hours old and produced from parthenogenically reproducing brood female population were used. Daphnia are issued from the breeding lab. The selection of the juvenile was daily realized by filtration.
The juvenile stemming at least from the second litter were used to start the test.

Culture conditions:
- Medium: Elendt M4
- Temperature: 20 °C ± 2
- Light/dark cycle: 16 h/8 h
- pH: Between 6 and 9
- Hardness (CaCO3): Between 140 and 250 mg.L-1
- Daily feeding: Pseudokirchneriella subcapitata and Chlorella vulgaris suspensions

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

20°C

pH:

7.2 - 8.2

Dissolved oxygen:

8.6 - 8.87

Nominal and measured concentrations:

nominal: 100 mg/L
measured (geo mean): 81.220 mg/L

Reference substance (positive control):

yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 81.22 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, both the EC50 after a 24 and 48-hour exposure period were estimated to be more than 81.220 mg test item.L-1.

Executive summary:

The aim of this study was to assess the effects of the test item on the mobility of Daphnia magna over a period of 48 hours under static conditions according to OECD Guideline 202 (April 2004).

The test item is a soluble substance under the test conditions and the test solution was prepared by directly dissolving the test item in the test solution.

The acute effects of the test itemin Elendt M4 test medium at the limit rate of 100mg test item.L^-1toDaphnia magnawere investigated under laboratory conditions.

Test item quantification was performed according to a validated analytical methodon aICS-5000+ from Thermo (Dionex) ion chromatography to measure test item concentrations at 0 and 48 hours.

Since the deviation from the target or measured initial concentration was not within the range of ± 20 %, statistical analysis of the results were based on the geometric mean exposure concentrations.

The study is valid since immobilization in the control was less than 10% (actual value: 0%), dissolved oxygen in the lowest concentration producing the maximum effect at the end of the test was ≥ 3 mg.L^-1(actual value: 8.6 mg.L^-1) and the EC₅₀with reference item over a period of 24 hours was between 0.6 and 2.1 mg.L^-1(actual value: 1 mg.L^-1).

Results were based on the loading rates and are the following:

 

	Titanium and potassium oxalate
EC50(24h)	> 81.220 mg test item.L^-1
EC50(48h)	> 81.220 mg test item.L^-1

EC = effect concentration.

Based on the number of immobilized daphnids, both the EC₅₀after a 24 and 48-hour exposure period were estimated to be more than81.220 mg test item.L^-1.

Description of key information

Under the test conditions, both the EC50 after a 24 and 48-hour exposure period were estimated to be more than 81.220 mg test item.L-1.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

81.22 mg/L

Additional information

Experimental study performed according to the OECD 202 guideline and under GLP conditions.

Validity criteria were successful.

The study is considered acceptable for that endpoint.