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EC number: 234-028-1 | CAS number: 10497-05-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source (i.e. manufacturer or supplier) and lot/batch number of test material:
Jiu Jiang Tinci Materials Techonology Co.
N200611031
- Purity, including information on contaminants, isomers, etc.:
99.28%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
Refrigeration (+2 to +8ºC)
- Stability and homogeneity of the test material in the vehicle/solvent under test conditions (e.g. in the exposure medium) and during storag:
Stable
- Stability in the medium, i.e. sensitivity of the test material to hydrolysis and/or photolysis:
Stable
- Solubility and stability of the test material in the solventehicle and the exposure medium:
Soluble in water.
- Reactivity of the test material with the incubation material used (e.g. plastic ware):
Non-reactive. - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- Chemical Concentration in Stock Solution
Stock solution 1: Macro Nutrients
NH4Cl
1.5 g/L
MgCl2.6H2O 1.2 g/L
CaCl2.2H2O 1.8 g/L
MgSO4.7H2O 1.5 g/L
KH2PO4 0.16 g/L
Stock solution 2: Iron
FeCl3.6H2O
64 mg/L
Na2EDTA.2H2O 100 mg/L
Stock solution 3: Trace Elements
H3BO3
185 mg/L
MnCl2.4H2O 415 mg/L
ZnCl2 3 mg/L
CoCl2.6H2O 1.5 mg/L
CuCl2.2H2O 0.01 mg/L
Na2MoO4.2H2O 7 mg/L
Stock solution 4: Bicarbonate
NaHCO3 50 g/L
The pH of the test medium after equilibration in air should be approximately 8.1. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name:
Raphidocelis subcapitata (formerly Pseudokirchneriella subcapitata)
- Strain:
ATCC® 22662
- Source (laboratory, culture collection):
American Type Culture Collection
P.O. Box 1549
Manassas, VA 20108, USA
- Age of inoculum (at test initiation):
5 days
- Method of cultivation:
These conical flasks are incubated between 21 and 25 °C (± 2°C) under a continuous illumination of 8000 ± 1600 Lux.
ACCLIMATION
- Acclimation period:
5 days
- Culturing media and conditions (same as test or not):
same
- Any deformed or abnormal cells observed:
none - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22.1-22.9℃
- pH:
- 7.63-7.98
- Nominal and measured concentrations:
- 0, 0.4, 1.2, 3.6, 10.8, 32.4, 97.2 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
flask
- Material, size, headspace, fill volume:
glass
- Aeration:
no
- Initial cells density:
10000 cells/mL
- Control end cells density:
70960 cells/mL
- No. of vessels per concentration (replicates):
3
- No. of vessels per control (replicates):
6
GROWTH MEDIUM
- Standard medium used:
yes
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
3
- Range finding study
- Test concentrations:
0, 0.01, 0.1, 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study:
%inhibition: 0, 0, 0, 2.58, 9.68, 100 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 20.62 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 25.94 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 38.39 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.44 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield (migrated information)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 4.45 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield (migrated information)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12.44 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield (migrated information)
- Results with reference substance (positive control):
- - Results with reference substance valid?
YES
- EC50:
ErC50=0.6450 mg/L
EyC50=0.2130mg/L
- Other: - Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 and EC10 of toxicity to aquatic algae and cyanobacteria is 38.39 and 20.62 mg/L, respectively.
Reference
Description of key information
The EC50 and EC10 of toxicity to aquatic algae and cyanobacteria is 38.39 and 20.62 mg/L, respectively.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 38.39 mg/L
- EC10 or NOEC for freshwater algae:
- 20.62 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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